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Brunauer, Linda S. – Journal of Chemical Education, 2016
A multiweek protein purification suite, suitable for upper-division biochemistry or biotechnology undergraduate students, is described. Students work in small teams to isolate the enzyme lactate dehydrogenase (LDH) from a nontraditional tissue source, mammalian blood, using a sequence of three column chromatographic procedures: ion-exchange, size…
Descriptors: Biochemistry, Biotechnology, Undergraduate Students, Science Instruction