Objective: To examine the effects of a mindfulness intervention on health-related quality of life in female Division I collegiate athletes. Participants 135 female collegiate athletes, ages 18-23 years Methods: Health related quality of life (HRQoL) was reported twice/year in approximately January and July from January 2017 to 2020 with the 12-question Veterans Rand survey (VR-12). Twenty-three of the participants received a 6-week, in-person, group mindfulness training in spring 2019, while 112 did not. Results: A significant interaction between time and mindfulness was identified with respect to the mental component score of the VR-12 of the VR-12 (MCS; [beta] = 3.86 ± 1 0.56, p = 0.012) but no significant relationships were identified with respect to time (pre-mindfulness: [beta] = -2.36 ± 1.38, p = 0.074), mindfulness (yes: [beta] = -2.26 ± 1.54, p = 0.14) or season (winter: [beta] = -0.84 ± 0.57, p = 0.14). With respect to the physical component score (PCS), no significant relationships were identified with respect to time (pre-mindfulness: [beta] = -1.09 ± 1.21, p = 0.37), mindfulness (yes: [beta] = 1.30 ± 1.31, p = 0.32), season (winter: [beta] = 0.50 ± 0.50, p = 0.32), or the interaction between time and mindfulness ([beta] = 0.35 ± 1.36, p = 0.80). Conclusions: Among female collegiate athletes, mindfulness training is associated with significant improvements in mental HRQoL, but not physical HRQoL.