This research study aims to investigate university student's critical thinking disposition (CTD). The sample includes 633 undergraduate students at different levels from Kasetsart University, Thailand. The analysis was based on convenience random sampling. The CTD instrument comprises 24 Likert scale. The subscales consisted of seven crucial dimensions: truth-seeking, open-mindedness, analyticity, systematicity, critical thinking self-confidence, inquisitiveness, and cognitive maturity. The cumulative percent of variance was 61.84%. Cronbrach's alpha for the overall instrument, the disposition toward critical thinking was 0.78. The findings revealed that critical thinking disposition has no distinctive correlation to significant difference towards gender and the different levels of university students significantly, particularly at the level of p<0.05.