The lateral flow immunoassay (LFIA) is a simple and cost-effective rapid diagnostic test designed for the on-site detection of disease biomarkers. In the context of introducing this technique to undergraduate biotechnology students, we propose a comprehensive 5 h laboratory experiment. This experiment covers various aspects, including the…

X-rays are such an important and familiar part of the electromagnetic spectrum, but is there a way to safely bring x-rays into your lecture? The demo team at the University of California at Berkeley seems to have managed this. They have an x-ray safe box that they use to produce a live x-ray image on a fluorescent screen. In this article, we…

Photocatalytic degradation of methylene blue was implemented as a laboratory experiment by the undergraduate students majoring in chemical education using graphene (GR)/TiO[subscript 2] as a catalyst. These chemistry preservice teachers synthesized a GR/TiO[subscript 2] composite and used it to degrade wastewater containing methylene blue under…

Electrophoresis is integral to analytical and biochemistry experiences in undergraduate education; however, fundamental principles of the method are often taught in upper-level laboratories through hands-on experiences. A laboratory activity is reported that teaches the concepts of electrophoretic mobility and electroosmotic flow. A single…

A set of inexpensive and pedagogically rich experiments focusing on S[subscript N]1, E1, and E2 reactions have been updated to include modern computational and spectroscopic analyses. The S[subscript N]1 experiment involves treatment of "tert"-amyl alcohol with hydrochloric acid to generate the corresponding alkyl chloride, which is used…

In this paper, we propose and describe a new approach, named BarcodingGO, to teach environmental DNA and bioinformatics concepts to undergraduate or graduate students in molecular biology-related fields. The learning pipeline proposed here aims to solve a simulated environmental monitoring problem, in which a biodiversity survey of a particular…

The use of symmetry is widespread in chemistry, as it is used for predictions of the number of allowed and forbidden absorptions in electronic, vibrational, and rotational spectroscopies; for predictions of the combination of atomic orbitals to produce molecular orbitals; and in many other chemical applications. One critical step in these…

Preceding even the Hartree-Fock method, molecular integrals are the very foundation upon which quantum chemical molecular modeling depends. Discussions of molecular integrals are normally found only in advanced and technical texts or articles. The objective of the present article is to provide less experienced readers, or students in a…

A significant consequence of the COVID-19 pandemic in higher education has been the almost universal switch to a completely online modality of teaching. This work evaluates the efforts taken in teaching a freshmen general chemistry course at IISER Tirupati to students in India during the pandemic. Instructors used a multipronged approach to…

The VSEPR model has well-established limitations in its ability to represent accurate molecular and electronic geometries of simple molecules, which can create a significant need for students to relearn structure and bonding concepts in organic chemistry. We present an alternate method for describing molecular geometries and electronic structures…

In the world of higher education, one of the struggles instructors face in the classroom is engaging students in the material. A second discussion in higher education pedagogy is how to weigh content versus activity in the science classroom. How should college teaching be set up when students now have every fact ever found at their fingertips on a…

NMR is a fundamental part of any undergraduate chemistry degree, and training students how to measure and interpret NMR spectra is essential for postgraduate research or chemistry-based careers. Traditionally, the undergraduate laboratory experience with NMR is restricted to sample submission for NMR analysis, which is typically conducted by a…

There are multiple ways to put science into a case. This column provides original articles on innovations in case study teaching, assessment of the method, as well as case studies with teaching notes. This month's issue discusses different ways of presenting science in case studies.

The real time PCR (qPCR) method provides a powerful method to assess levels of particular species of DNA. When combined with reverse transcription (RT-qPCR) it is the predominate technique to measure expression of gene transcripts. While this approach is very powerful, particular care must be taken in the design of the primers to facilitate…

Two of the most fundamental skills that students acquire in introductory undergraduate physics laboratory courses are how to accurately plot data and interpret the physical meaning of linear graphs. We redesigned our graphing skills lab with an open-ended approach designed to promote inquiry in graphical analysis and to address specific areas of…