An undergraduate laboratory experiment for the synthesis of biologically significant bis-4-hydroxycoumarins using a new catalyst is described here. An experimental setup plan, monitoring the reaction by TLC, isolation of the product, safe laboratory handling procedures, and significance of data reproducibility of the result are the main learning…

The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In…

Recombinant protein expression and site-directed mutagenesis of target genes have demonstrated an increasing importance in the fields of molecular biology, biochemistry, biotechnology, and medicine. By using the flavodoxin of the model cyanobacterium "Anabaena" sp. PCC 7120 as a laboratory tool, we designed a comprehensive laboratory…

Laboratory exercises for undergraduate biochemistry students are described in which changes in sugar content during fermentation of the trendy beverage kombucha are analyzed by three methods: thin layer chromatography, a 3,5-dinitrosalicylic acid assay, and a standard commercial blood glucose meter. Each of the three analyses can be completed in a…

In order to introduce the concepts of biocatalysis and its utility in synthesis to organic chemistry students, a multistep synthesis of endogenous cannabinergic ligand 2-arachidonoylglycerol (2-AG) was tailored for use as a laboratory exercise. Over four weeks, students successfully produced 2-AG, purifying and characterizing products at each…

Monohybrid crosses are taught in biology labs across the country using peas and corn. Students studying monohybrid crosses using zebrafish ("Danio rerio") would encounter a new model organism, and those interested in human or veterinary medicine would have increased interest. To this end, our college has implemented a laboratory exercise at the…

Laboratory work is considered essential in promoting students' learning of science and of scientific inquiry. What the students perceive as important to learn from a regular laboratory exercise is probably affected by the teacher's objectives. We study the extent to which one teacher's objectives are fulfilled during lab work, and how…

Personalized medicine refers to medical care that involves genetically screening patients for their likelihood to develop various disorders. Commercial genome screening only involves identifying a consumer's genotype for a few single nucleotide polymorphisms. A phenotype (such as an illness) is greatly influenced by three factors: genes, gene…

I present a laboratory procedure for illustrating transcription, post-transcriptional modification, gene conservation, and comparative genetics for use in undergraduate biology education. Students are individually assigned genes in a targeted biochemical pathway, for which they design and test polymerase chain reaction (PCR) primers. In this…

A central theme of the "National Science Education Standards" is teaching science as an inquiry process, allowing students to explore an authentic problem using the tools and skills of the discipline. Research indicates that more active participation by the student, which usually requires higher-order thinking skills, results in deeper learning.…

Inquiry-driven lab exercises require students to think carefully about a question, carry out an investigation of that question, and critically analyze the results of their investigation. Here, we describe the implementation and assessment of an inquiry-based laboratory exercise in which students obtain and analyze novel data that contribute to our…

The creative application of low-cost, readily available materials and techniques promotes inclusion and provides accessibility in the classroom and laboratory for students who are blind or have low vision. Difficulties encountered by these students include operation of laboratory equipment, execution of ordinary laboratory procedures, and use of…

Contemporary science standards stress the importance of highlighting inquiry in the science classroom as one way of learning key concepts. One critical question that needs to be addressed for a range of instructional practices is how opportunities to learn are conceptualized so that students can engage and learn the critical ideas and practices…

This manual provides practical advice on the appropriation, conservation, and documentation of a marine biological reference collection, in response to needs expressed by Mediterranean Arab countries. A reference collection is defined as a working museum containing a series of specimens with which biologists are able to compare their own material.…

Smaller amounts of materials are used in organic chemistry experiments as a means of improving air quality in the laboratory. Presents a preliminary study of the emissions from selected processes and the mixing of these emissions within the laboratory atmosphere. (JN)