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Showing 1 to 15 of 569 results Save | Export
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Vimoshini Pragash; Meran Keshawa Ediriweera – Biochemistry and Molecular Biology Education, 2025
The Folch method is a commonly employed, simple biochemical procedure used to extract fat from food items and biological samples in biochemistry laboratories. The iodine number of fat reflects its degree of unsaturation. In the present study, the iodine number of fat extracted using the Folch method from cow milk, goat milk, chicken egg yolk and…
Descriptors: Biochemistry, Science Laboratories, Laboratory Procedures, Science Experiments
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Alison Bates; Kathryn M. Williams; Ann E. Hagerman – Biochemistry and Molecular Biology Education, 2025
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…
Descriptors: Undergraduate Students, Science Instruction, Laboratory Experiments, Kinetics
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Bolduc, Kathryn C.; McCollough, Shannon L.; Stoeckman, Angela K. – Biochemistry and Molecular Biology Education, 2023
Undergraduate laboratory courses are vital for both enhancing student learning and preparing students for their future careers. Despite their importance, laboratory courses are often met with a lack of enthusiasm from students. For pre-health students specifically, laboratory courses are commonly seen as part of a check-list that needs to be…
Descriptors: Biochemistry, Science Laboratories, Laboratory Training, Majors (Students)
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Naoto Shirasu; Shin'ichiro Yasunaga – Biochemistry and Molecular Biology Education, 2024
Here, we propose a laboratory exercise to quickly determine single nucleotide polymorphisms (SNPs) in human "alcohol dehydrogenase 1B (ADH1B)" and "aldehyde dehydrogenase 2 (ALDH2)" genes involved in alcohol metabolism. In this exercise, two different genotyping methods based on polymerase chain reaction (PCR), namely…
Descriptors: Genetics, Biochemistry, Students, Science Instruction
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Maurye, Praveen; Basu, Arpita; Jana, Chayna; Devi, Manoharmayum Shaya; Kumari, Kavita; Naskar, Malay – Biochemistry and Molecular Biology Education, 2022
Gel electrophoresis (GE) is the most preferred and adapted technique for the separation and identification of biological molecules like proteins/peptides and nucleic acids from diverse types of organisms. All over the world, researchers, educators, and students aspiring to work in biochemistry and molecular biology disciplines use the…
Descriptors: High School Students, Biochemistry, Molecular Biology, Laboratory Procedures
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Maurizio Costabile; Bradley Simpson; Jasmina Turkanovic; Bernard P. Hughes – Biochemistry and Molecular Biology Education, 2024
This article details the outcome of a joint reflective approach undertaken by the authors to identify common difficulties experienced by 2nd-year undergraduate Biochemistry students in laboratory classes. Difficulties experienced in laboratories can affect the development of hand skills, an understanding of how to correctly operate laboratory…
Descriptors: Biochemistry, Science Laboratories, Instructional Effectiveness, Undergraduate Study
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María Florencia Mosquillo; Gonzalo Scalese; Felipe Castro; Leticia Pérez-Díaz – Natural Sciences Education, 2024
An effective, practical approach for incorporating molecular biology tools to biology and biochemistry students is described. For 6 weeks, students are exposed to bacterial transformation, plasmid DNA purification and analysis, parasite transfection, ectopic expression of fluorescent proteins, fluorescence microscopy, and flow cytometry analysis.…
Descriptors: Active Learning, Student Projects, Science Education, Molecular Biology
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Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna – Biochemistry and Molecular Biology Education, 2021
The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In…
Descriptors: Biochemistry, Cytology, Metabolism, Science Laboratories
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Katharine Hubbard; Marlena Birycka; Maisie-Elizabeth Britton; Joseph Coates; Isla Delphine Coxon; Chloe Hannah Jackson; Casper Leigh Nicholas; Tyler M. Priestley; J. J. Robins; Paula R. Ryczko; Talia Salisbury; Megan Shand; George Snodin; Beth Worsley – Journal of Biological Education, 2024
Providing hands-on practical education without access to laboratories during the COVID-19 pandemic has required creativity and innovation. In this paper, co-authored by academic staff and students, we describe an at-home mobile phone-based 'spectrophotometer' experiment used in an introductory undergraduate biology course. Using colour picker…
Descriptors: Hands on Science, Science Laboratories, Distance Education, Telecommunications
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Louise Castro de Jesus; Sophia Lincoln Cardoso de Azevedo; Rafaela Moraes Pereira de Sousa; Ana Beatriz Matos; Salim Kanaan; José Carlos Pelielo de Mattos; Lidia Maria Amorim – Journal of Biological Education, 2025
Isoenzymes are variant forms of the same enzyme activity that exhibits different tissue distribution. When cells are damaged, intracellular content leaks into the blood stream, making them potential biochemical markers. Undergraduate health sciences students are expected to interpret laboratory findings of serum levels of biochemical markers and…
Descriptors: Science Laboratories, Biochemistry, Health Sciences, Allied Health Occupations Education
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Nicola´s Andre´s Saffioti; Carla Angelani; Jose´ Mari´a Delfino; Silvia Ferna´ndez Villamil; Gabriela Elena Go´mez; Irene Mangialavori; Mari´a Florencia Pignataro; Pablo Julio Schwarzbaum – Journal of Chemical Education, 2023
Metabolic Control Analysis (MCA) is a systems biology approach that measures the control of a metabolic step on the overall flux of a metabolic pathway. In undergraduate courses in biochemistry, learning of MCA can be encouraged by experimental activities demonstrating practical applications of the MCA theory. In this work, we present a laboratory…
Descriptors: Biochemistry, Metabolism, College Science, Undergraduate Students
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Ce´sar Pastrana-Pineda; Luis J. Melgoza-Rami´rez; Erandi M. Islas-Flores; Wendy L. Go´mez-Monsiva´is; Jero´nimo Gonce; Noe´ Lo´pez; Jose´ B. Limo´n-Gonza´lez; Jose´ S. Rodri´guez-Zavala; Emma Saavedra; Javier A. Belmont-Di´az – Journal of Chemical Education, 2025
The study of energy metabolism is fundamental in multiple disciplines, such as biochemistry, bioengineering, biomedicine, etc. Traditionally, in high school and college level courses, students are taught energy metabolism (glycolysis, Krebs cycle, and oxidative phosphorylation) through diagrams of metabolic pathways that depict the transformation…
Descriptors: Science Instruction, Science Laboratories, Biochemistry, Energy
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Andrea C. Bardales; Quynh Vo; Dmitry M. Kolpashchikov – Journal of Chemical Education, 2024
It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which…
Descriptors: Active Learning, STEM Education, Scientific Concepts, Concept Formation
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Matthew R. Anderson; Cammi J. Dargatz; Tuhina Banerjee; Natasha M. DeVore – Biochemistry and Molecular Biology Education, 2024
Green fluorescent protein has long been a favorite protein for demonstrating protein purification in the biochemistry lab course. The protein's vivid green color helps demonstrate to students the concept(s) behind affinity or ion exchange chromatography. We designed a series of introduction to biochemistry labs utilizing a thermostable green…
Descriptors: Science Education, Biochemistry, Laboratory Experiments, Color
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Mark A. Chrisman; Michael J. Goldcamp; Alexis N. Rhodes; Jared Riffle – Journal of Chemical Education, 2023
This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel­(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…
Descriptors: Science Laboratories, Science Experiments, Laboratory Experiments, Science Instruction
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