Despite the growing awareness of the importance of plant secondary metabolites in insect-plant interactions, undergraduate degree content in agronomy and biology generally does not provide a clear concept to students in relation to secondary metabolite induction of plant defences, implying that students do not obtain a good understanding of the…

The chemical experiment is designed for undergraduates majoring in chemistry, energy, and environment. The intended purpose is that the students will comprehend the reaction mechanism of photocatalytic reduction of CO[subscript 2] through hands-on experiments and deepen their understanding of sustainable energy technologies. More importantly, the…

Students in environmental sciences, ecology, or wildlife management are often reluctant to acquire training in basic laboratory techniques. To advocate the importance of this training, we reviewed literature in the fields of biological and environmental science for one frequently used technique--centrifugation--and evaluated whether centrifugation…

This paper describes an inquiry-based integrated laboratory project for green synthesis, characterization, and applications of silver and gold nanoparticles. In this project, students were able to choose their choice of plant extract as reducing and stabilizing agents, and the students also designed their own experiments. This course gave the…

Mass spectrometry (MS) has become an important tool in studying biological systems. One application is the identification of proteins and peptides by the matching of peptide and peptide fragment masses to the sequences of proteins in protein sequence databases. Often prior protein separation of complex protein mixtures by 2D-PAGE is needed,…

Field-based undergraduate geography courses provide numerous pedagogical benefits including an opportunity for students to acquire employable skills in an applied context. This article presents one unique approach to teaching geographic field methods using paleoecological research. The goals of this course are to teach students key geographic…

This experiment is designed to teach entry-level, nonchemistry-major students some basic organic chemistry through the procedure for making cosmetics. The experiment combines facial toner preparation with distillation techniques to create a stimulating learning environment. During the experiment, students perform a distillation to collect some…

We describe a laboratory exercise developed for a cell biology course for second-year undergraduate biology majors. It was designed to introduce undergraduates to the basic molecular biology techniques of Western blotting and immunodetection coupled with the technique of tissue printing in detecting the presence, relative abundance, and…

The introduction of dwarfing traits into crops was a major factor in increased grain yields during the "Green Revolution." In most cases those traits were the consequence of altered synthesis or response to the gibberellin (GA) plant hormones. Our current understanding of GA synthesis and physiology has been facilitated by the characterization of…

A simple tissue-printing procedure to determine the tissue location of the endogenous enzymes polyphenol oxidase and peroxidase in a variety of vegetables, fruits, and mushrooms is described. In tissue printing, cell contents from the surface of a cut section of the tissue are transferred to an adsorptive surface, commonly a nitrocellulose…

Can you imagine a class where students cover the content with each other rather than simply listening to the professor's lecture? Can you envision students developing their own laboratory investigations and quizzing each other weekly to check for understanding? Well, that's pretty much how the major science organizations across the nation are…

Plants defend themselves against pathogens via the expressions of disease resistance (R) genes. Many plant R gene products contain the characteristic nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. There are highly conserved motifs within the NBS domain which could be targeted for polymerase chain reaction (PCR) cloning of R…

Many topics in the secondary science classroom can be difficult to introduce to students in a manner that fully engages them, especially when presented using traditional teaching methods. However, with a little innovation and an emphasis on inquiry, even dry subjects can be presented in an appealing way. The authors developed an inquiry-based…

More than 90% of vascular plants in terrestrial ecosystems form symbiotic root-fungal associations known as mycorrhiza; in Mediterranean biomes all known mycorrhizal types co-exist. However, the understanding of mycorrhizal diversity and structure and their ecophysiological impact in soil productivity and conservation has been shown to be very…

An HPLC undergraduate experiment on the analysis of traditional Chinese medicine (TCM) has been developed. Two commonly used herbs ("glycyrrhizae radix" and "cinnamomi ramulus") are studied. Glycyrrhizin, cinnamic acid, and cinnamaldehyde are chosen as markers for the herbs. The dried herbs in their natural state and a TCM…