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Direct linkR. A. Paggi; M. C. Ferrari; M. Cerletti; M. I. Giménez; T. S. Schwarz; A. Marchfelder; R. E. De Castro – Biochemistry and Molecular Biology Education, 2025
Perturbation of gene expression using RNA interference (RNAi) or CRISPR interference (CRISPRi) is a useful strategy to explore the function of essential genes. In the archaeon "Haloferax volcanii," the CRISPR-Cas system has been adapted as a CRISPRi tool to silence the expression of specific genes. We developed a laboratory class (LC) to…
Descriptors: Undergraduate Students, Laboratory Experiments, Science Instruction, Biochemistry
