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Quorum Sensing Inhibition Evaluation Method: An Experiment-Based Microbiology Laboratory Course
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Ângelo Luís; Fernanda Domingues – Biochemistry and Molecular Biology Education, 2025
Bacteria have developed a cell-to-cell communication system called quorum sensing (QS), allowing them to regulate group behavior and synchronize the expression of virulence factors, responsible for increasing their infection capacity and resistance to antimicrobials. Although the control of microbial infections through the inhibition of microbial…
Descriptors: Inhibition, Evaluation Methods, Science Experiments, Microbiology
Adapting Chiral Gas Chromatography into Existing Undergraduate Laboratories to Emphasize the Importance of Stereochemistry
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Nicholas Griffin; Marshall Ritchie; Tyler Lynn; Kate Dear; Tyler Christian John Deutsch; Leigha Dillard; Kenneth Overway; Barnabas Otoo – Journal of Chemical Education, 2024
Owing to the critical role of stereochemistry in biotechnology, medicine, and industry, it must be well represented in undergraduate lab curricula. To achieve these stereochemical laboratory requirements without sacrificing existing lab techniques, we modified two standard laboratory experiments to include chiral GC analysis as follows. (1) The…
Descriptors: College Science, Undergraduate Students, Chemistry, Laboratory Procedures
Electrochemical Nucleic Acid Biosensor on a Microfluidic Chip to Understand the Coupling between Electrochemistry and Microfluidics
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Marie-Charlotte Horny; Martina Freisa; Claire Poujouly; Pedro Gonzalez-Losada; Jeremy Le Gall; Thi-Hong-Nhung Dinh; David Bouville; Isabelle Le Potier; Antoine Pallandre; Jean Gamby – Journal of Chemical Education, 2024
Teaching graduate students the entanglements of the interdisciplinary field of electrochemical DNA biosensors in microfluidics can be challenging, as it involves multiple fields of research strongly interacting with each other. Yet, electrochemical biosensors coupled with the improved hydrodynamics of microfluidics allow for simpler, more…
Descriptors: Graduate Students, Chemistry, College Science, Science Instruction
Lake Pigments and Thin-Layer Chromatography of Natural Dyes: Paired Experiments for Nonmajors
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Simeen Sattar – Journal of Chemical Education, 2024
Lakes are artists' pigments made from colorants bound to an inert substrate, usually hydrated aluminum oxide. Before the late 19th century, lakes were made from natural pigments extracted from plants and insects, extracted either directly from the dyestuff or indirectly from waste materials generated in the manufacture of dyed textiles. The first…
Descriptors: Color, Science Experiments, Laboratory Experiments, Science Education
NMR in the Advanced Inorganic Lab. II. Introduction to Quadrupolar Coupling: What to Do When the "n + 1" Rule and Pascal's Triangle Fail
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T. Leon Venable – Journal of Chemical Education, 2024
As an introduction to quadrupolar effects in NMR spectroscopy, students use low field ([superscript 1]H, 60 MHz), benchtop [superscript 13]C NMR spectroscopy to contrast the spin-spin coupling behavior of [superscript 13]C to the dipolar [superscript 1]H and quadrupolar [superscript 2]D in familiar solvents C(H/D)Cl[subscript 3], C(H/D)[subscript…
Descriptors: Inorganic Chemistry, Science Laboratories, Scientific Concepts, Spectroscopy
A Project-Based Learning Mode for Science Students: Fluorescent Protein Expression in Trypanosomes
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María Florencia Mosquillo; Gonzalo Scalese; Felipe Castro; Leticia Pérez-Díaz – Natural Sciences Education, 2024
An effective, practical approach for incorporating molecular biology tools to biology and biochemistry students is described. For 6 weeks, students are exposed to bacterial transformation, plasmid DNA purification and analysis, parasite transfection, ectopic expression of fluorescent proteins, fluorescence microscopy, and flow cytometry analysis.…
Descriptors: Active Learning, Student Projects, Science Education, Molecular Biology
Exploring Cation-Exchange Membranes: Synthesis, Characterization, and Real-World Applications in a Multidisciplinary Laboratory
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Qi Sun; Qing Guo; Xiuhui Zuo; Sai Wang – Journal of Chemical Education, 2024
This laboratory experiment offers a research-style experience designed for senior undergraduate students in a multidisciplinary laboratory setting. The experiment focuses on the synthesis, characterization, and application of a cation-exchange membrane made of COF-SO[subscript 3]Na/PAN for ion separation. By participating in this practical…
Descriptors: Undergraduate Study, Science Instruction, Chemistry, Theory Practice Relationship
Developing a Threshold Concept Assessment Rubric: Using the Johnstone's Triangle Framework for Understanding Intermolecular Forces
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Simbarashe Nkomo; Alia Bly – Journal of Chemical Education, 2024
In undergraduate science education, laboratory courses stand as essential cornerstones of experiential learning. Chemistry laboratory courses offer students unique hands-on experiences that bridge the gap between theoretical knowledge and practical application. The journey through the undergraduate chemistry curriculum is paved with a series of…
Descriptors: Undergraduate Students, College Science, Chemistry, Hands on Science
A Workshop to Enrich Physiological Understanding through Hands-On Learning about Mitochondria-Endoplasmic Reticulum Contact Sites
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Zer Vue; Chia Vang; Celestine N. Wanjalla; Andrea G. Marshall; Kit Neikirk; Dominique Stephens; Sulema Perales; Edgar Garza-Lopez; Heather K. Beasley; Annet Kirabo; Yelena Janumyan Doe; Desmond Campbell; Letimicia Fears; Ahmad Alghanem; Estevão Scudese; Beverly Owens; Derrick J. Morton; Clintoria R. Williams; Zachary Conley; Hinton Antentor Jr. – Advances in Physiology Education, 2024
Physiology is an important field for students to gain a better understanding of biological mechanisms. Yet, many students often find it difficult to learn from lectures, resulting in poor retention. Here, we utilize a learning workshop model to teach students at different levels ranging from middle school to undergraduate. We specifically designed…
Descriptors: Workshops, Physiology, Science Education, Hands on Science
Evidence for Validity and Reliability of a Research-Based Assessment Instrument on Measurement Uncertainty
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Gayle Geschwind; Michael Vignal; Marcos D. Caballero; H.? J. Lewandowski – Physical Review Physics Education Research, 2024
The Survey of Physics Reasoning on Uncertainty Concepts in Experiments (SPRUCE) was designed to measure students' proficiency with measurement uncertainty concepts and practices across ten different assessment objectives to help facilitate the improvement of laboratory instruction focused on this important topic. To ensure the reliability and…
Descriptors: Measurement, Ambiguity (Context), Scientific Concepts, Physics