The COVID pandemic forced higher educational institutions to transition from in-person lectures to online lectures. In Bangladesh, this transition was steep, with no prior experience of this mode of delivery. This study aimed to understand and compare the experiences of students, academic staff and support staff within the area of veterinary science in Bangladesh, when comparing in-person and online lectures. We conducted a mixed methods survey to learn about the perceived experiences, strengths and limitations of each mode of teaching from students and staff. Our findings highlighted that students, academic staff and support staff all preferred in-person lectures when compared to online lectures. However, the extent of preference varied, with support staff strongly favouring the in-person mode of delivery, followed by academic staff and then students. Overall, these findings are essential to aid policymakers and university authorities on some of the strengths and barriers of in-person and online lectures.