The Ouchterlony double immunodiffusion technique is used as a teaching tool for studying immune responses and exemplifying differences in antigen-antibody reactions. Although commonplace in undergraduate labs, standardized commercial kits limit learning experiences because they have fixed modalities of use, a low shelf-life, and impose budgetary…

Teaching graduate students the entanglements of the interdisciplinary field of electrochemical DNA biosensors in microfluidics can be challenging, as it involves multiple fields of research strongly interacting with each other. Yet, electrochemical biosensors coupled with the improved hydrodynamics of microfluidics allow for simpler, more…

Despite the growing awareness of the importance of plant secondary metabolites in insect-plant interactions, undergraduate degree content in agronomy and biology generally does not provide a clear concept to students in relation to secondary metabolite induction of plant defences, implying that students do not obtain a good understanding of the…

The substitution of carbonyl compounds with liquid bromine as a brominating agent is the most commonly employed synthesis method for a-bromoacetophenone and its derivatives. However, liquid bromine possesses certain drawbacks, such as volatilization, high toxicity, strong corrosion, and poor reaction selectivity, which limit its application and…

Calculating analytical uncertainties as a part of method validation is a relevant aspect of field and laboratory practices in instrumental analytical chemistry subjects, which usually require complex algorithms. This work describes the development and didactic use of an automatic and straightforward informatics tool, implemented in an Excel macro,…

Chemical spillage has been measured for undergraduate students undertaking a laboratory practical designed to help develop their chemical handling skills. There was a low correlation found between the amount of chemical spilled and the ability to accurately carry out the primary goal of the experiment, which was to measure the concentrations of…

Titration is an important experimental skill that every chemistry educator and learner must master. Titration is often the method of choice in the science laboratory courses in secondary and postsecondary schools. During acid-base titration, chemistry learners are always told about salt formation. However, a practical visualization of the salt…

Descriptive statistics involves summarizing and organizing data so that they can be easily understood. Even though these are basic and simple concepts, many applied science students have misconceptions about their use in applied experiments in the laboratory. Students usually receive limited or no training in how to understand the meaning of the…

Both nanopore-based DNA sequencing and CRISPR/Cas-based gene editing represent groundbreaking innovations in molecular biology and genomics, offering unprecedented insights into and tools for working with genetic information. For students, reading, editing, and even writing DNA will be part of their everyday life. We have developed a laboratory…

Undergraduate laboratory courses can provide opportunities for students to participate in science practices. This requires rethinking both curricula and instruction. Science practice--based courses require students to be positioned as epistemic agents, implying a shift in instructor role. Teaching assistants (TAs) are the primary instructors for…

This paper reports an immersive virtual reality lab (iVRLab) training environment that offers college students an immersive and embodied experience in engineering lab work. The iVRLab provides a simple and safe environment for students to learn the complex lab operations to process and prepare silicon wafers. It features highly embodied…

Prior to the challenges imposed by the COVID-19 pandemic, anatomy practical sessions at Trinity College Dublin involved eight to 10 students per donor station, rotating between digital learning, anatomical models/osteology, and dissection activities for three hours weekly. To maintain cadaveric participation in the anatomy laboratory while…

Few laboratory exercises exist that introduce undergraduate students to both the concepts and instrumentation used in rotational spectroscopy. Because of this gap in undergraduate laboratory training, a teaching laboratory for the pure rotational spectroscopy of benzonitrile has been developed and reported. Owing to benzonitrile's recent discovery…

The CRISPR/Cas9 system is widely used for editing genes in various organisms and is a very useful tool due to its versatility, simplicity, and efficiency. To teach its principles to post-graduate students we designed a laboratory activity to obtain and analyze PDS3 mutants in "Arabidopsis thaliana" plants consisting of: 1) Design of…

Laboratory course activities often focus on "cookbook style," so their activities have not yet integrated with scientific thinking as an essential aspect. In this research, the laboratory experience of microbial analysis techniques was redesigned using alternative inquiry to involve students in isolating and characterising yeast to make…