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ERIC Number: EJ1300805
Record Type: Journal
Publication Date: 2021-Jun
Pages: 7
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Available Date: N/A
Extracting and Quantifying Deuterated Bisphenol A from "Planaria" Using Gas Chromatography/Mass Spectrometry: An Integrative Laboratory Experiment
St. John, Pamela M.; Kienzle, Scott D.; Flood, Brianna M.; Moody, Troy V.; DePaola, Nicole F.; Mass, Spencer
Journal of Chemical Education, v98 n6 p2090-2096 Jun 2021
With the enormous reliance on plastics in the world today, bisphenol A, an additive used in the synthesis of many plastics, such as polycarbonate, has been found almost everywhere in our environment including in soil, in natural waters, and inside organisms including humans. A multidisciplinary experiment is presented that involves using flatworms ("Planaria"), which are model organisms common to undergraduate biology courses, to study the extent of retention or absorption of bisphenol A following exposure. "Planaria" were exposed to micromolar concentrations of deuterated bisphenol A in order to distinguish the amount of the compound retained from the (undeuterated) bisphenol A that is present as a contaminant in the environment. Liquid extraction was used to remove retained deuterated BPA from "Planaria," and the extract was derivatized and quantified using gas chromatography/mass spectrometry. This experiment is geared toward students in a laboratory research course or independent study experience. It would also be appropriate in analytical or environmental chemistry courses to help students understand how contaminants in our environment brought about from manufacturing processes can be measured and quantified. Students will learn standard techniques in extraction and analysis to quantify their findings.
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A
Author Affiliations: N/A