Postsecondary science faculty face challenges in balancing the engagement of undergraduates and concomitantly ensuring they gain and retain knowledge, either in standard lectures or labs as well as in outdoor activities. Designing on-campus trails with interpretive signs may provide a unique avenue to inform students across majors of local biodiversity and tie in concepts related to ecology, organismal biology, and conservation. In this article, we evaluate the efficacy of course-specific interpretive signs deployed around a small on-campus natural area to engage first-year students. We assessed students' overall engagement level and retention of information conveyed across specific signs via a student-guided nature walk where students examined signs and answered both a pre- and post-activity survey, rated their favorite sign, and provided a short reflection on this outdoor activity. Students' responses indicated that they retained general information on specific signs, most notably regarding reptiles and what types of mammals are found around campus. Overall, students rated this activity as engaging, with 87.7% combined agreeing or strongly agreeing that signs helped them learn about the biodiversity and ecosystems on campus.