Background: Aphasia, a communication disorder mainly resulting from stroke, poses challenges to the meaningful interactions between healthcare professionals and people with aphasia (PWA). Little is known about the knowledge and skills of Cypriot healthcare professionals when interacting with PWA in clinical settings. This study explores the knowledge and skills of Cypriot healthcare professionals in communicating with PWA. Method: A total of 245 Greek-speaking Cypriot healthcare professionals completed the Greek version of the Health Professionals and Aphasia Questionnaire (HPAQ). Participants' knowledge and skills were assessed in five categories: Knowledge, Skills, Behaviour and Emotions, Practice, and Environment. Demographic data were analysed through linear regression and ANOVA to identify predictors of HPAQ scores. Results: Participants' mean HPAQ score was 94.57 (SD = 28.1). Higher educational levels (master's degree) and the Speech and Language Therapy profession significantly predicted higher HPAQ scores. Moderate frequency of interaction with PWA (1-20 times/week) was also associated with improved knowledge of aphasia and better communication skills when interacting with PWA. Conclusion: Speech and Language Therapists exhibited higher competency in interacting with PWA compared to other healthcare professionals, highlighting their specialised training. The findings emphasise the importance of education, experience, and interaction frequency in enhancing healthcare professionals' ability to communicate effectively with PWA in clinical settings. To address identified gaps, targeted interventions, such as Communication Partner Training, are recommended to improve communication strategies and patient outcomes in Cypriot healthcare settings.