This study assessed the knowledge, feelings, and life experiences of students in public middle schools in the Brazilian Amazon toward aquatic mammals by applying semi-structured interviews, with a qualitative-quantitative approach. A total of 241 students, from all genders, aged between 12 and 14 years old, from four public schools of Pará State were interviewed. The data were transcribed and analyzed in an inductive way, in dialog with the literature of scientific education and ethnozoology. This methodology was developed and validated during the doctorate research of the first author of this paper. The students showed a rich knowledge, feelings, and life experiences regarding the Amazonian aquatic mammals, those related to biological, morphological, behavioral, ecological, and taxonomic aspects, as well as the threat to these species. The research reveals the need for schools of the region to develop and use didactic resources and strategies for intercultural dialogue in science classes, favoring the development of reflective attitudes in students and promoting decision-making favorable to the conservation of aquatic mammals.