This study is part of a research about the use of ICT resources by Higher Education teachers to teach, evaluate and research (UICT-TER). The aim, in this case, is the creation and validation of a measuring instrument on the use of ICT resources based on the UICT-TER model. After the preparation and application of the instrument to a sample of 867 Higher Education teachers in the Spanish territory, the reliability, content validity, comprehension and construct validity were analyzed. In the initial creation of the instrument, three dimensions corresponding to a total of 24 items were established, achieving a final version of 16 items. Regarding content validity, the experts (n = 4) positively assessed the construct, dimensions and items of the questionnaire. The reliability analysis through Cronbach's alpha were highly satisfactory and in relation to the Confirmatory Factor Analysis, the results found a good fit of the model. In addition, the instrument had external evidence of validity through different descriptive analyses with the variables gender, type of university (public-private), employment relationship (full-part-time), area of knowledge of the teaching staff (Science and Engineering-Architecture, Health Sciences, Art-Humanities and Social-Legal Sciences). Taking into account all the results obtained, the instrument created meets the psychometric properties required to be considered a valid and reliable instrument to measure the use of ICT resources according to the UICT-TER model in the Higher Education.