The current study is aimed to determine if Turkish IT pre-service teachers' acceptance of tablet PCs is within the framework of the Technology Acceptance Model. The research was patterned as a phenomenological study which is among the qualitative research methods. Participants were eight pre-service teachers studying in Trakya University, Faculty of Education, Computer Education and Instructional Technologies Department during the 2011-2012 academic year. A semi-structured interview, an activity list for tablet PCs, the students' reflective journal and the researcher's reflective journal were used to collect the data. Research data were analyzed via content analysis. Findings showed that IT pre-service teachers have technology acceptance towards tablet PCs which in turn directs in a positive way their attitude and behavioral intention to use. Nonetheless, various challenges concerning the hardware, software and communication may have negative effects on IT pre-service teachers' technology acceptance of tablet PC.