When the risk factors causing infertility are examined in detail, most appear preventable. Primary prevention studies are considered among the most effective ways of reducing risk factors. This study aimed to determine the effects of an infertility prevention psycho-education programme on university students' infertility knowledge and attitudes. The psycho-education programme consisted of ten 90-minute sessions. The study employed a Solomon four-group experimental design with random assignment. Participants (n = 52) were university students enrolled in an Education programme in a state university in the west of Turkey. The groups were similar in terms of socio-demographic characteristics and infertility-related risky behaviours. To determine the effect of the intervention and its impact on infertility knowledge and attitudes, the researchers performed a two-way ANOVA. The results of the analyses showed that the infertility prevention psycho-education programme had a significant positive effect on participants' infertility knowledge and attitudes, free from the psycho-education programme's pre-post-test sensitivity.