This study was aimed to determine the opinions of nursing students about the usage of YouTube in content of operating room nursing lecture. A descriptive study was conducted with 144 3rd grade students. The data were collected with "Sociodemographic Characteristics and Technology Use Form" and "Evaluation Form of Students' Opinions on the Use of YouTube Videos in the Operating Room Lecture". Data were analyzed by number percentage, mean and standard deviation. 68.9% of the nursing students stated that the integration of YouTube videos to be good. 97.9% of the students stated that they were pleased that the lecture was video-aided. The rate of effective, intelligibility and adequate use of YouTube videos was found between 59.7% and 74.3%, 69.5% and 90.3%, 89.6% and 95.2%, respectively. Nursing students indicated that they were pleased with the integration of YouTube videos into the Operating Room Nursing lecture and they found the videos to be effective, clear and sufficient.