Examines the influence of enzyme and substrate concentrations, pH, temperature, and inhibitors on catalytic activity. Follows the influence of different phosphate concentrations in the growth medium on enzyme activity. Studies regulation of enzyme synthesis by repression. Includes methodology for six experiments. (MVL)

Offers several advantages over other possibilities as the enzyme of choice for a student's first exposure to a purification scheme. Uses equipment and materials normally found in biochemistry laboratories. Incorporates several important biochemical techniques including spectrophotometry, chromatography, centrifugation, and electrophoresis. (MVL)

Presents a hand-out given to students at the University of Canterbury, New Zealand, concerning expectations and guidelines for finishing laboratory reports. Topics covered are accuracy, data collection, and write-up format that includes introduction, materials utilized, procedures, results, discussion, conclusion, tables and illustrations, units…

An experiment that uses the recombinant plasmid pX1108 to illustrate restriction mapping is described. The experiment involves three restriction enzymes and employs single and double restriction enzyme digestions. A list of needed materials, procedures, safety precautions, results, and discussion are included. (KR)

Describes a program to support lectures on analytical centrifugation, and to illustrate the manner in which useful analytical data about macromolecules can be obtained from simple centrifugation studies without the need for mathematical expertise. Discusses the background, methods, calculations, and results involved in this activity. (CW)

Illustrates the scope of experimental investigations for biochemistry education in high school biology and chemistry courses. Gives a brief overview of biochemistry experiments with proteins, enzymes, carbohydrates, lipids, nucleic acids, vitamins, metabolism, electron transport, and photosynthesis including materials, procedures, and outcomes.…

An activity that introduces students to the correct handling of bacterial recombinants, antibiotic sensitivity testing, insertional inactivation, plasmid DNA isolation, restriction endonuclease digestion, agarose gel electrophoresis, Southern blotting, hybridization, and autoradiography is presented. A list of needed materials, procedures, safety…

Describes an experiment on the use of problem-based learning. (Author/YDS)

Explains the procedures used to determine the regulatory properties of yeast pyruvate kinase. Involves a partial purification using PEG precipitation that can be done in one laboratory period with simple equipment. (DDR)

Proposes an examination of the motives in having biochemistry students perform practical laboratory exercises. Suggests that a typical course should be viewed as a progression of skills development culminating in a final year, open-ended research project that demands that the skills be used competently and efficiently. (DDR)

Describes an instructional experiment that was designed to illustrate the use of periodate oxidation in sugar analysis, and the consequence that the absorbance of mixtures of non-interacting substances is additive. (TW)

Describes a simple method for purifying catalase for the study of proteins. Procedures are systematically and diagramatically presented. Also identifies polyacrylamide gel electrophoresis, kinetic studies, and apparent molecular weight determination as possible techniques to be used in studying proteins. (ML)

Describes a science activity designed for undergraduate biochemistry classes. Provides background, a list of materials, experimental procedures, and the results of this experiment. (CW)

Presents a practical exercise that enables students to measure flux control coefficients in a metabolic pathway in vitro, to verify the summation property, and to compare the differences in the distribution of these coefficients in different steady states. Discusses background, materials, methods, and results of the activity. (CW)

Describes the construction of an inexpensive apparatus for separating proteins by polyacrylamide gel electrolysis in the presence of sodium dodecyl sulphate. (TW)