New suitable approaches were investigated to visualize the photodynamic inactivation (PDI) of bacteria immobilized on agar surfaces. The PDI capacities of a cationic photosensitizer (5,10,15,20-tetra(4-N,N,N-trimethylammoniumphenyl)porphyrin) and an anionic photosensitizer (5,10,15,20-tetra(4-sulfonatophenyl)porphyrin) were analyzed on a typical…

Described is a teaching method for connecting relative acidity among various species by the use of familiar references. Considered are teaching the acidity of alpha-hydrogens of ketones, alkylation of amides and imides, and others. (CW)

Describes a one-semester course designed to teach first year graduate students how to extract, interpret, evaluate, and use the information provided by an X-ray crystallographic crystal structure determination. Presents a course outline and discusses the treatment of crystal composition, crystallographic parameters, molecular geometry and…

Proposes a series of experiments that integrate analytical techniques in order that students are able to compare, based on their laboratory results, the relative reliabilities of the most common therapeutic drug monitoring methods. Discusses materials, procedures, and results of three experiments on the determination of drug concentration by…

Explains that triglycerides consist of a mixture of different compounds, depending on the total number of fatty acid constituents. Details the method and instrumentation necessary for students to analyze a vegetable oil for its triglyceride content. Describes sample results. (CW)

Describes a purification process through affinity chromatography necessary to obtain specific blood group glycoproteins from erythrocytic membranes. Discusses the preparation of erythrocytic membranes, extraction of glycoprotein from membranes, affinity chromatography purification, determination of glycoproteins, and results. (CW)

Presented is a classification of polymers according to physical condition. Considered are molecular structure and arrangements. Described are demonstrations using polarized light and the mechanical properties of polymers. (CW)

Describes an experiment that provides a fundamental understanding of the kinetics of the enzyme papain. Discusses background, materials, procedures and results. Mentions analogous experiments that can be conducted with enzymatic contact-lens cleaning solutions. (CW)