This document, which lists the biotechnology competencies identified by representatives from biotechnology businesses and industries as well as secondary and post-secondary educators throughout Ohio, is intended to assist individuals and organizations in developing college tech prep programs that will prepare students from secondary through post-secondary associate degree programs for employment as technicians in the biomedical, environmental, pharmaceutical, and other biotechnology related industries, such as bioinformatics. The technical competencies are listed in the following categories: demonstrate scientific method; conducting experiments; laboratory safety and maintenance; instrument analysis; chemical materials handling and sampling; physical properties measurement; biohazard storage, handling, and disposal; basic microbiology; biochemical technology; molecular biology technology; cell culturing; protein bioseparation methods; fermentation technology; microbiology for biotechnology; bioethics; water and wastewater treatment operations; environmental science; environmental assessments; introduction to industry; technical writing and documentation; computer applications for biotechnology; and a bioinformatics strand for database administration, data warehousing, and statistics. The competencies, which are separated into essential competencies needed to ensure a minimal level of employability and recommended competencies, are organized by instructional units and include suggestions as to when students should be introduced to and proficient at them. Appendices include a list of technical competency profile (TCP) panel members and a pathway template. (MO)