Data collected in many biology laboratory classes are on ratio or interval scales where the size interval between adjacent units on the scale is constant, which is a critical requirement for analysis with parametric statistics such as t-tests or analysis of variance. In other cases, such as ratings of disease or behavior, data are collected on…

Students learn to examine the distributional assumptions implicit in the usual t-tests and associated confidence intervals, but are rarely shown what to do when those assumptions are grossly violated. Three data sets are presented. Each data set involves a different distributional anomaly and each illustrates the use of a different nonparametric…

Null distributions of permutation tests for two-sample, paired, and block designs are simulated using the R statistical programming language. For each design and type of data, permutation tests are compared with standard normal-theory and nonparametric tests. These examples (often using real data) provide for classroom discussion use of metrics…