Tofu, a traditional Chinese food, is now popular worldwide. However, few people notice the chemistry that is involved in its production. To shed light on this, we have designed a simple demonstration for lower-level undergraduates in organic chemistry or biochemistry courses to help them understand the chemistry principles that underlie the…

Enzymes, enzymatic kinetics, and enzyme inhibition are topics covered in undergraduate biochemistry textbooks, having perceptible relevance to everyday life. The experimental use of enzymes involved in basic physiologic processes may be of utmost pedagogic interest because they bring evidence on how a significant number of drugs are used to…

Traditional undergraduate biochemical laboratory curricula are confronted with challenges in improving student-learning outcomes while incorporating exciting and thought-provoking research-based laboratories due to limitations with time, increased student enrollment, and high-cost laboratory resources. However, with specialized biochemical…

Optimization of experimental conditions in chromatography is recognized as a wear-and-tear process involving hundreds of experiments in order to maximize the number of separated compounds for each sample. The critical thinking skills and practices entailed cannot be achieved with students in a conventional lecture class. This report provides a…

Structure--activity relationships are foundational concepts in biochemistry and drug design. Despite their importance, it can be difficult to create and execute laboratory activities that allow students to directly observe the interactions between small-molecule drugs and their biomolecular targets. Here, we report an activity that investigates…

Students encounter antioxidants in many areas of their daily life. Antioxidants play a crucial role in cosmetics, in nutritional or dietary supplements, as additives for the preservation of food, and as a natural component in fruit and vegetables. Accordingly, antioxidants are believed to promote health through the prevention of oxidative stress…

A process for generating and writing an individual research proposal with or without access to advanced experimental techniques is described herein. This process was first included in a glycoconjugate biochemistry course to increase student interaction with more areas of the field, but the process was designed to be applicable elsewhere as well.…

Every day we deal with chemistry in various forms, but sometimes we don't see that. An example is biosensors that help us to evaluate health conditions, monitor environmental pollution and ensure food freshness. The most well known (and most widely sold) is the biosensor for blood glucose measurement, used every day by people with diabetes.…

Course specific learning outcomes are an important tool to define the scope of a course and can be very helpful when designing experiments and assessments. With slight modification, these learning outcomes can serve as a guide when transitioning to the distance learning format especially in courses with a traditional lab. Here we present such an…

Protein engineering represents a modern approach to generate novel proteins for the different fields of biotechnology. Here, we report about an 8-day laboratory activity in which students generate enzyme variants to degrade the herbicide glyphosate. The students conduct a true research experiment in an important field (bioremediation by novel,…

An upper-division undergraduate- or graduate-level laboratory experiment, challenging students to identify bovine serum albumin, equine myoglobin, bovine calmodulin, and bovine cytochrome c, uses four methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): linear positive, in-source decay, peptide…

Colleges and educational institutions throughout the globe have adapted a pseudo-online educational model. This is also evident in health sciences courses designed for biochemical and chemical education. Biochemistry (along with general, organic and analytical) education is significant in the basic prerequisite education of undergraduate health…

A six-laboratory session biofilm-focused biochemistry laboratory module is described. Through just six four-hour laboratory sessions over three weeks, students first master background knowledge and fundamental experimental techniques to explore questions involving bacterial biofilms at the interface of microbiology and biochemistry and then…

A chemistry course designed as an elective for chemistry, biochemistry, biology, and pharmaceutical science majors is described. The course is designed for second- through fourth-year undergraduate students. The prerequisite is first-semester, second-year undergraduate organic chemistry with the second semester of the organic sequence as a…

A laboratory experiment introducing the concept of chemical bioconjugation of proteins to undergraduate students in a therapeutically relevant context was developed. Initially, students installed an aldehyde functionality into a protein via the oxidation of the N-terminal threonine residue of the cholera toxin subunit B (CTB) protein, which was…