Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

The pharmaceutical and medicine manufacturing industry has become the largest industrial sector for the employment of chemists, indicating a need for experiments with a pharmaceutical sciences context in the undergraduate chemistry curriculum. In the pharmaceutical industry, testing drug dissolution is a key analytical task for solid oral dosage…

An integrated system comprising a commercial high-performance liquid chromatography HPLC (except autosampler) equipped with an external multichannel autofeeding module was developed to accomplish interference-free separation and kinetic tracking for the H[subscript 2]O[subscript 2]-thiourea reaction, which is proposed as a lab experiment for…

A laboratory class was developed and evaluated to illustrate the synthesis of metal nanoclusters (NCs) and to explain their photoluminescence properties for the case of silver. The described experiment employs a synthetic protocol that consists of two sequential phases in a single reaction pot: the reduction of silver ions into plasmonic silver…

Millions of people do not have access to clean drinking water; thus, cost-efficient water treatment systems are vital. Chemists, environmentalists, technicians, and engineers will be the professionals making breakthroughs in this industry. This laboratory experiment aims to introduce undergraduate students to the removal of pollutants from water…

This practical is aimed at students who have been introduced to the tableting process, drug delivery systems, and dissolution. The student can design and manufacture a tablet investigating the effect of excipients and their role in tablet design and then generate a release profile of the tablet by using a dissolution apparatus, flow-through cell,…

In this guided inquiry experiment, students extract catecholase enzyme from apples to catalyze the oxidation of catechol. They follow the reaction using the UV--vis absorbance of the "p"-benzophenone produced to determine the Michaelis-Menten kinetic parameters. Students make selected experimental choices within a structured framework…

The redox reaction between dioxovanadium(V) and oxalate is proposed as a suitable system to analyze the kinetics of a twostep reaction mechanism using UV-vis spectroscopy. First, the spectra of dioxovanadium(V) and its reduced counterpart oxovanadium(IV) are compared and explained using the crystal field theory. The change in absorbance can be…

A laboratory experiment that exposes students to fundamental concepts in kinetics and statistical thermodynamics to study systems of great current interest in nanotechnology by employing a foundational technique in physical chemistry is described. A modified commercial flash photolysis spectrometer was used to study photoinduced isomerization…

In this laboratory experiment, students explore the aquatic photochemical fate of ranitidine and cimetidine, two common pharmaceutical pollutants found in wastewater. It provides an engaging environmental context for students to develop knowledge of reaction kinetics and photochemistry as well as skill in using analytical instrumentation. This…

This article describes a straightforward approach to deliver an enzyme assay and kinetics laboratory via online delivery methods in the time of COVID-19.

This paper describes an undergraduate biochemistry laboratory module consisting of a set of experiments designed around a purification scheme for bovine serum albumin (BSA). Students purify BSA from cow plasma by a combination of salt and acetone precipitation, equilibrium dialysis, ion exchange, and size exclusion chromatography. Students use the…

Most biochemical transformations involve more than one substrate. Bisubstrate enzymes catalyze multiple chemical reactions in living systems and include members of the transferase, oxidoreductase, and ligase enzyme classes. Working knowledge of bisubstrate enzyme kinetic models is thus of clear importance to the practicing biochemist. However,…

In an article in this journal, it was shown that air resistance could never be a significant source of error in typical free-fall experiments in introductory physics laboratories. Since projectile motion is the two-dimensional version of the free-fall experiment and usually follows the former experiment in such laboratories, it seemed natural to…

Supersonic free-fall jumps are excellent examples of kinematics in the context of drag. They have attracted a lot of media, public, and scientific interest. In 2012, Felix Baumgartner jumped from a height of approximately 38.969 km. During his flight he reached a top speed of 373 m/s, becoming the first human to travel faster than the speed of…