Plant extracts and their components have been reported to have antimicrobial properties against phytopathogens. Although such properties could be harnessed for the development of new pathogen control strategies, there are no standardised methods for their assessment in different extracts. The laboratory exercise presented here involves the use of…

An undergraduate laboratory experiment has been developed for the synthesis of a reactive cross-linker that is useful in various highly visual demonstrations of organic reactivity, click chemistry, and biobased polymer synthesis. A triazolinedione-based cross-linker, which is known as a useful reagent for click chemistry, can be obtained from…

Poinsettia ("Euphorbia pulcherrima") contains anthocyanins that dissolve in water and exhibit color changes due to changing pH. This property makes the poinsettia extract a useful acid-base indicator for pH demonstrations. When combined with common household materials, this experiment can be used as an alternative low-cost tool in…

The CRISPR/Cas9 system is widely used for editing genes in various organisms and is a very useful tool due to its versatility, simplicity, and efficiency. To teach its principles to post-graduate students we designed a laboratory activity to obtain and analyze PDS3 mutants in "Arabidopsis thaliana" plants consisting of: 1) Design of…

Drought is a major environmental stress factor that affects the growth and development of plants. All plants have to maintain the reactive oxygen species within certain levels for normal cellular homeostasis by means of their antioxidant systems, which can be classified as enzymatic and non-enzymatic. Plants under drought stress generate an excess…

Active learning pedagogies offer opportunities to increase student engagement and interest in plants. The Mystery Plant Project is a hands-on, semester-long active learning pedagogy I developed for an introductory, undergraduate Plant Biology course. Students investigate various aspects of their mystery plant species using observations, and…

A laboratory experiment is described in which students synthesize silver nanospheres and transform them into other anisotropic morphologies (decahedral and disks) through a photochemical process. The photochemical transformation was followed by visible spectroscopy, and the resulting morphologies were analyzed using scanning electron microscopy.…

Rhizobacteria that colonize plant roots and promote plant growth are referred to as plant growth-promoting rhizobacteria, and this can stimulate plant growth either indirectly or directly. Volatile organic compounds (VOCs) emitted by rhizobacteria have the capacity to promote plant growth as well as perform biocontrol of fungal pathogens. The…

With the benefits of low cost, energy efficiency, and environmental protection, photocatalytic hydrogen production is considered to be the most promising technology for the development of a clean and sustainable society. However, the utilization of photocatalytically generated hydrogen is not very common yet. Therefore, tertiary education urgently…

Secondary metabolite compounds contained in each plant genus have certain characteristics, which is true also of the flavonoid in the genus "Artocarpus." In the experiment described here, third-year undergraduate students performed the isolation and characterization of cycloartocarpin from the roots of "Artocarpus altilis"…

Plant nitrogen nutrition is an essential topic in biology that should be included in scientific education. Nitrogen availability is one of the primary limiting factors for plant growth, and these organisms are the primary support for the pluricellular terrestrial life, since they are the fundamental group of autotrophs. For this reason, the use of…

Systems thinking is highly desirable for re-imagining chemistry education, which will help in the development of an integrated and sustainable approach that takes into account the interdependence of a system under study with other components of the ecosystem rather than practicing a fragmented approach. Thus, to develop a systems' perspective into…

The practice of RNA isolation in undergraduate experimental courses is rare because of the existence of robust, ubiquitous and stable ribonucleases. We reported here modifications to our original protocol for RNA isolation from plant tissues, including the recovery of nucleic acids by ethanol precipitation at 0 degrees C for 10 min and the…

Allelopathy plays crucial roles in invasive plant viability and agricultural production systems. However, there is no well-established hands-on learning activity to teach the concept of allelopathy. Nor is there an activity which allows students to gain knowledge about glucosinolates and their corresponding enzyme, myrosinase, which are present in…

A two-week experiment is presented in which students can observe the impact of nanoparticles on the concentration of chlorophyll in plants. First-year students in an introductory nanotechnology laboratory course and a general chemistry laboratory course synthesized silver nanoparticles and then exposed stalks of "Egeria densa" ("E.…