This paper describes an inquiry-based integrated laboratory project for green synthesis, characterization, and applications of silver and gold nanoparticles. In this project, students were able to choose their choice of plant extract as reducing and stabilizing agents, and the students also designed their own experiments. This course gave the…

Field-based undergraduate geography courses provide numerous pedagogical benefits including an opportunity for students to acquire employable skills in an applied context. This article presents one unique approach to teaching geographic field methods using paleoecological research. The goals of this course are to teach students key geographic…

This experiment is designed to teach entry-level, nonchemistry-major students some basic organic chemistry through the procedure for making cosmetics. The experiment combines facial toner preparation with distillation techniques to create a stimulating learning environment. During the experiment, students perform a distillation to collect some…

Can you imagine a class where students cover the content with each other rather than simply listening to the professor's lecture? Can you envision students developing their own laboratory investigations and quizzing each other weekly to check for understanding? Well, that's pretty much how the major science organizations across the nation are…

Plants defend themselves against pathogens via the expressions of disease resistance (R) genes. Many plant R gene products contain the characteristic nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. There are highly conserved motifs within the NBS domain which could be targeted for polymerase chain reaction (PCR) cloning of R…

Many topics in the secondary science classroom can be difficult to introduce to students in a manner that fully engages them, especially when presented using traditional teaching methods. However, with a little innovation and an emphasis on inquiry, even dry subjects can be presented in an appealing way. The authors developed an inquiry-based…

More than 90% of vascular plants in terrestrial ecosystems form symbiotic root-fungal associations known as mycorrhiza; in Mediterranean biomes all known mycorrhizal types co-exist. However, the understanding of mycorrhizal diversity and structure and their ecophysiological impact in soil productivity and conservation has been shown to be very…

Conventional sellotape techniques for fungal mounting produce interference patterns when using Normarsky microscopy. A technique is described which overcomes this problem and produces a permanent mount with a completely clear background. (Author/JN)

Describes methods for isolating, purifying, and analyzing plant hormones (molecules involved in plant growth regulation and development). The presentation reflects the historical development of analyses, beginning with bioassays and ending with novel immunochemical assays. (JN)

Describes an inexpensive and convenient way to solve the problem of maintaining the proper humidity needed in incubation chambers. (JN)

Clearing is a simple and rapid technique in which 75 percent lactic acid is used to remove pigments and cytoplasmic contents of fresh leaves, enabling microscopic view of various internal leaf layers. Procedures for using the technique (which helps students gain a more thorough understanding of plant anatomy) are given. (DH)

Two methods for examining leaf structure are presented; both methods involve use of "superglue." The first method uses the glue to form a thin, permanent, direct replica of a leaf surface on a microscope slide. The second method uses the glue to examine the three-dimensional structure of spongy mesophyll. (JN)

Describes a method for the extraction and bioassay of natural germination inhibitors, requiring only inexpensive equipment and minimal experimental skill. The method has been used to demonstrate qualitative/quantitative differences in germination inhibitor levels in a variety of different fruits or in different tissues within a single fruit.…

Describes a method suitable for quantitative studies of plant water status conducted by high school or college students and the calculation of the relative water content (RWC) of a plant. Materials, methods, procedures, and results are discussed, with sample data figures provided. (CS)

Procedures are outlined for a laboratory exercise in which students use a gravimetric method to determine the rate of transpiration in sunflower seedlings. Discusses the data in terms of the effectiveness of stomatal openings, mechanisms for water movement in plants, and the role of transpiration in the environment. (DC)