The study of energy metabolism is fundamental in multiple disciplines, such as biochemistry, bioengineering, biomedicine, etc. Traditionally, in high school and college level courses, students are taught energy metabolism (glycolysis, Krebs cycle, and oxidative phosphorylation) through diagrams of metabolic pathways that depict the transformation…

Chemistry experiments involving biological aspects are in high demand. This laboratory exercise blends biochemistry and physical chemistry, illustrates key concepts of the virus capsid surface, and is designed for a chemistry laboratory course for students majoring in biology in college. The experimental part provides a mastery of…

Isothermal titration calorimetry (ITC) is the method of choice for thermodynamic binding profiling. As a label-free in-solution technique, ITC experiments do not require modified or immobilized proteins or ligands and hence can be performed under close-to-native environmental conditions. It directly follows the released heat of the molecular…

Tofu, a traditional Chinese food, is now popular worldwide. However, few people notice the chemistry that is involved in its production. To shed light on this, we have designed a simple demonstration for lower-level undergraduates in organic chemistry or biochemistry courses to help them understand the chemistry principles that underlie the…

Biochemistry primarily focuses on the non-metal chemical elements carbon, oxygen, nitrogen, hydrogen, sulfur, and phosphorus in the four groups of building blocks (sugars, lipids, amino acids, and nucleotides) and the corresponding macromolecules. However, at least 10 essential chemical elements of life are metals. This article discusses the…

The COVID-19 pandemic affected a large range of in-person education activities in Colombia. This created great limitations in academic performance for students with reduced access to communication technologies and deepened the educational gaps in the country. This was particularly true for sciences such as biochemistry. In Colombia, molecular…

Given the severe pollution caused by traditional plastics, biodegradable plastic products (BPPs) have received great attention and are appearing in people's lives in the form of packaging bags, tableware, etc. Whether a wide variety of BPPs can truly degrade depends on the environment. Aerobic composting is the arbitration method to evaluate the…

In a typical undergraduate biochemistry course, two main educational objectives include (1) understanding and applying how genetic mutations can influence protein structure and function, and (2) examining metabolic pathways of biomolecules to study cellular storage and fuel. Many times, these topics can seem disparate to students; therefore, we…

Sanger sequencing, also known as dideoxy sequencing, is a widely used method for DNA sequencing, particularly for cloned plasmids and clinical samples. This technique requires a combination of essential biochemistry skills, such as a chain-termination reaction, gel electrophoresis, and fluorescence detection. Unfortunately, there is a lack of…

DockIT is an interactive molecular docking tool suitable for teaching students about concepts related to drug-receptor interaction. Its most unique feature is the ability to model both local and global conformational change in the receptor based on information derived from the trajectory of a molecular dynamics (MD) simulation. The workshop…

Biochemistry is a data-heavy discipline, yet teaching students to work with large datasets is absent from many undergraduate Biochemistry programs. Ensuring that future generations of students are confident in tackling problems using big data first requires that educators become comfortable teaching big data skills. The activity described herein…

In this work extensive misconceptions of university students'--from nutrition area--about the metabolism of carbohydrates (CHM) in the human organism have been documented. The results lead to consider their difficulties concerning the learning of a complex set of imbricated biochemical models involved. Pursuant to these considerations, three…

Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

In collaboration with educators and researchers, we created an online resource called Phase Separation 101 to help undergraduate students understand the basics of liquid-liquid phase separation, an emerging and complex concept in cell biology for which visual resources are still scarce. This work presents the workflow and visual communication…

An understanding of structure-function relationships in proteins is essential for modern biochemical studies. The integration of common freely accessible bioinformatics tools available online with the knowledge of protein-engineering tools provide a fundamental understanding of the application of protein structure-function for biochemical…