In this laboratory exercise, students have an opportunity to evaluate the potential endocrine disrupting abilities of environmental chemicals of their choice using human cell culture. Over the course of 9 weeks, students learn how to aseptically handle and manipulate cells, perform and analyze a cytotoxicity assay and an enzyme-linked immunosorbent assay. Following completion of the module, the majority of students reported large or very large gains not only in laboratory performance, but also in understanding of the scientific literature and research process, as well as scientific communication skills. The student survey results imply that this authentic laboratory experience improves students' scientific literacy and prepares them for future careers in science.