Most textbooks and lecturers present Michaelis-Menten kinetics using the equation v = V[subscript max][S]/(K[subscript m] + [S]). There are advantages to presenting this relationship in a slightly different form, namely v = V[subscript max]/{1 + (K[subscript m]/[S])}. We articulate advantages for single-substrate reactions and extend the formalism…

Transcription is the critical first step in expressing a gene, during which an RNA polymerase (RNAP) synthesizes an RNA copy of one strand of the DNA that encodes a gene. Here we describe a laboratory experiment that uses a single assay to probe two important steps in transcription: (1) RNAP binding to DNA, and (2) the transcriptional activity of…

We are living in the Big Data era, and yet we may have serious troubles when dealing with a handful of kinetic data if we are not properly instructed. The aim of this paper, related to enzyme kinetics, is to illustrate how to determine the K[subscript m] and V[subscript max] of a michaelian enzyme avoiding the pitfalls in which we often fall. To…

Virtual laboratory simulations can contribute to the educational objectives related to practical classes, especially in situations of online or hybrid teaching. We present a proposal for laboratory activity involving the quantification of proteins with the biuret reaction and measurement by visible light spectrometry. The student will be able to…

Analogies are useful tools instructors can use to help make challenging concepts less abstract by drawing connections to familiar contexts. In this paper we provide an overview of the various analogies published in the education literature that are situated in the context of enzyme kinetics, including narrative-based analogies (analogies intended…

Lab courses are a significant component of biochemistry and molecular biology (BMB) education. In teaching the labs, we combine established techniques with novel approaches. Lab formats have also moved from traditional cookbook style labs to guided inquiry to course-based undergraduate research experiences (CUREs), where faculty bring their own…

Ensuring currency with trends, knowledge, and understanding of teaching and learning is essential for all educators. Researching learning and teaching is an enormous field which can range from examining the practical impact of new classes to research into the processes of learning. The "Publishing in Education" conference session…

This brief review explores the ever-increasing role that technological affordances may play in the 21C biochemistry and molecular biology curriculum. We consider the need to develop digital and creative fluencies in our students and the importance of creativity and visualization in learning science. The potential of virtual reality (VR) platforms…

A simple one-dimensional [superscript 1]H NMR experiment that quantifies protein bound to gold nanoparticles has been developed for upper-division biochemistry and physical chemistry students. This laboratory experiment teaches the basics of NMR techniques, which is a highly effective tool in protein studies and supports students to understand the…

Accreditation of academic programs and recognition of student degrees provide academic institutions a measure of a set of community agreed upon standards. These can aid pedagogical change, support faculty to successfully engage students in their discipline and to provide a mechanism to maintain standards. Several professional scientific societies…

The physical and chemical properties of Milk of Magnesia provide examples of chemical concepts and opportunities for investigative practical work for secondary chemistry students of all ages. Basing the lesson on the contents of that familiar blue bottle, which is probably lurking somewhere in a cupboard in most households, also showcases the role…

To meet complex ethical challenges in the engineering profession, students need ethics learning experiences that are integrated systematically across the engineering curriculum. Sustaining systematic changes in the engineering curriculum also calls for processes that respect and engage the engineering faculty. This paper reports the work to date…

The practical work described here is designed for third-year bachelor students in Life Sciences attending a kidney physiology course. It illustrates how urinary biochemistry can be used for a medical diagnosis. Students have to measure glucose, proteins, and creatinine concentrations in three simulated urine samples. First, they independently…

The recent more-than-human turn has increased interest in writing about relations between humans and other animals. In addition, scholars have called for a need to complement the animal turn with a turn to microbes. Microbes entangle all life in relations and participate in processes of living and dying, but thus far, they have been largely absent…

Medical laboratory technology major was set up to meet rapid development of science and medical research technology in 2013. Students majoring in medical laboratory had learnt a lot of techniques distributed among different specialized courses. But, they did not understand why they had to learn these techniques and how they were applied in a…