Presents a protocol that uses reverse phase chromatography for the separation of chloroplast pigments. Provides a simple and relatively safe procedure for use in teaching laboratories. Discusses pigment extraction, chromatography, results, and advantages of the process. (JRH)

Describes a lab designed to analyze the organic compounds found in an ordinary fast-food lunch. (ZWH)

Discusses simple and safe techniques that can be used in the educational laboratory to study hemoglobin. Discusses the spectral properties of hemoglobin, spectral-absorbence curves of oxyhemoglobin and carboxyhemoglobin, tracking the conversion of oxyhemoglobin to methemoglobin, and changing from the oxyhemoglobin to deoxyhemoglobin conformation.…

Describes a bioassay laboratory exercise designed to introduce students to both acute and chronic bioassay procedures. Reinforces ecological principles and provides opportunities for students to use knowledge learned in the classroom in a realistic and ecologically-relevant situation. Contains 11 references. (JRH)

Background information, list of materials needed, procedures used, and discussion of typical results are provided for an experiment on the thin layer chromatography of chlorophyll pigments. The experiment works well in high school, since the chemicals used are the same as those used in paper chromatography of plant pigments. (JN)

Presents a laboratory experiment on determining the molecular weight of an unknown molecule by using gel filtration chromatography. Describes the procedure and lists required materials. (YDS)

Presented is an approach to the study of nutrient cycling in the school laboratory. Discussed are obtaining, processing, and incubating samples; extraction of ions from soil; procedures for nitrate and ammonium analysis; data analysis; an example of results; and other aspects of the nitrogen cycle. (CW)

Describes the use of the computer in the laboratory for data acquisition and analysis--specifically a method to measure the respiration rate in aquatic organisms. Demonstrates the use of computer-interfaced pH measurement in the introductory biology laboratory. (PR)