Previous research suggests that parents of children with ASD experience greater marital conflict than parents of children with neurotypical development (NTD). This study examines how "relational uncertainty" is associated with "taking conflict personally" among parents of children with or without ASD. Parents of children with ASD (N = 298) and parents of children with NTD (N = 316) completed an online survey. They reported their relational uncertainty, recalled a conflict related to their child, and completed measures of taking conflict personally. The study provides evidence that spouses' experiences of relational uncertainty may be associated with conflict about topics related to their child. In addition, experiences of self uncertainty may have a greater impact for the parents of children with ASD than parents of children with NTD.