Plant extracts and their components have been reported to have antimicrobial properties against phytopathogens. Although such properties could be harnessed for the development of new pathogen control strategies, there are no standardised methods for their assessment in different extracts. The laboratory exercise presented here involves the use of…

The curriculum for undergraduate Agricultural Engineering does not usually include much laboratory practice. In order to address this, here we propose the design of a practical class on legume-rhizobia symbiosis, using an interdisciplinary approach that takes elements from botany, agriculture, and microbiology. The students perform an assay to…

Graduates in biology or genetics learn Mendel's laws and Hardy-Weinberg equilibrium as students, and they know, use, and sometimes teach these concepts. However, it is unusual to learn about stochastic processes during the graduate studies of these topics, although the applications of Markov chains in the fields of genetics or biology are…

Enzyme-Linked Immunosorbent Assay (ELISA) is a versatile and widely used technique to detect and quantify small molecules in various clinical and analytical applications. It is also an effective tool for demonstrating the specificity between antibody and antigen to students across diverse disciplines. However, undergraduate laboratories often face…

In the world of higher education, one of the struggles instructors face in the classroom is engaging students in the material. A second discussion in higher education pedagogy is how to weigh content versus activity in the science classroom. How should college teaching be set up when students now have every fact ever found at their fingertips on a…

The development and evaluation of a two-session laboratory class, based on Geneswitch technology and sex determination in the fruit fly Drosophila melanogaster, is described. Geneswitch system allows conditional control of gene expression. A laboratory exercise has been devised for sophomores in order to illustrate how Geneswitch technology can be…

Mutation, diversity, natural selection and the biology of human pathogens (including antibiotic resistance) are key features of the biosciences curriculum at A Level and undergraduate study. Few resources exist to allow students to engage with these topics in an interactive manner. This paper describes an interactive, online simulation of mutation…

All the cells of higher organisms have the same DNA but not the same proteins. Each type of specialised cell that forms a tissue has its own pattern of gene expression and, consequently, it contains a particular set of proteins that determine its function. Here, we describe a laboratory exercise addressed to undergraduate students that aims to…

A phylogenetics exercise is incorporated into the "Introduction to biocomputing" course, a junior-level course at Savannah State University. This exercise is designed to help students learn important concepts and practical skills in molecular phylogenetics through solving a real-world problem. In this application, students are required to identify…

Field experiments are at the core of ecology, yet rarely find their way into education, probably due to a combination of technical challenges and risks associated with fieldwork. This may compromise student education and perception of the subject, making interesting, readily manageable experiments extremely important. We describe how the daily…

Citrus fruit (oranges, tangerines, grapefruit or lemons) purchased in a grocery store can be experimentally infected with readily-available sources of "Penicillium digitatum" to demonstrate the four basic steps of Koch's postulates, also known as proof of pathogenicity. The mould is isolated from naturally-infected citrus fruit into pure culture…

Reconstructing phylogenies from nucleotide sequences is a challenge for students because it strongly depends on evolutionary models and computer tools that are frequently updated. We present here an inquiry-based course aimed at learning how to trace a phylogeny based on sequences existing in public databases. Computer tools are freely available…

Monoclonal antibodies have provided an exciting addition to the "armory" of the molecular biologist and immunologist. This article discusses briefly the concept of, techniques available for, production of, and possible uses of monoclonal antibodies. (Author)

Conventional sellotape techniques for fungal mounting produce interference patterns when using Normarsky microscopy. A technique is described which overcomes this problem and produces a permanent mount with a completely clear background. (Author/JN)

To contribute usefully to a lecture, slides containing tabulated or diagrammatic textual information need to be simple and legible. Such slides can be made by following a few straightforward rules and techniques. Several of these rules and techniques are provided. (JN)