Plant extracts and their components have been reported to have antimicrobial properties against phytopathogens. Although such properties could be harnessed for the development of new pathogen control strategies, there are no standardised methods for their assessment in different extracts. The laboratory exercise presented here involves the use of…

Despite the growing awareness of the importance of plant secondary metabolites in insect-plant interactions, undergraduate degree content in agronomy and biology generally does not provide a clear concept to students in relation to secondary metabolite induction of plant defences, implying that students do not obtain a good understanding of the…

Providing hands-on practical education without access to laboratories during the COVID-19 pandemic has required creativity and innovation. In this paper, co-authored by academic staff and students, we describe an at-home mobile phone-based 'spectrophotometer' experiment used in an introductory undergraduate biology course. Using colour picker…

Isoenzymes are variant forms of the same enzyme activity that exhibits different tissue distribution. When cells are damaged, intracellular content leaks into the blood stream, making them potential biochemical markers. Undergraduate health sciences students are expected to interpret laboratory findings of serum levels of biochemical markers and…

Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to…

We developed a fluorescence microscopy lesson geared towards high school students. Our goals included demonstrating how fluorescence microscopy works and illustrating the localisation of key cellular structures/organelles in the model organism, budding yeast. A trained student teacher/researcher on our team took the microscopy images and…

Among the efforts directed at remediating contaminated sites, the use of immobilized microorganisms is an appealing alternative because it offers several advantages over the use of free cells, including protecting cells from the toxic effects of hazardous compounds and increasing their survival and metabolic activity. "Pseudomonas…

The contamination of wastewater with metals and the use of biotechnologies to remove them is commonly discussed in theoretical biology, microbiology and biotechnology classes aimed at undergraduate students. This work introduces an easy, economical and safe practical laboratory class related to this topic. The practice consists in performing batch…

Forward genetics involves the identification of an unknown DNA sequence (genotype) associated with the expression of that sequence (phenotype). This is often done by generating variants (mutants) of that feature. One of the most practical methods is transposon mutagenesis, which facilitates the genotype-phenotype association through a sequence…

Wheat powdery mildew is an important fungal pathogen of wheat with an obligatory biotrophic lifestyle (a parasite that can only develop on a living host). We investigated the genetics of this host-pathogen interaction by using phenotyping and PCR assays to detect genes in both wheat and powdery mildew, which are known determinants of the outcome…

Universities play an important role in society and in providing solutions for its problems. Environmental pollution is one of the most serious problems facing the world today. Thus, universities are increasingly including subjects related to the environment and its conservation both in their educational and research programmes. Generally,…

Live cell imaging is a standard technique in experimental biology that enables the observation of isolated cells and tissue slices in real time and the testing of cellular responses to changes in buffer composition. However, most live cell imaging devices require the use of dedicated microscopes and/or specialised stage adaptors, and come at a…

In recent years the possibility of observing by microscopy the dynamic activity of living cells has been largely pursued. We have developed a low-cost (~ 260 euros) on-stage cell incubator for inverted optical microscopes. This device allows to keep cells in good conditions for their survival and proliferation. The device is based on the use of…

"Sinorhizobium meliloti" is a bacterium of great agroeconomic importance because of its ability to fix atmospheric nitrogen in symbiotic association with alfalfa ("Medicago sativa") roots, and it is often used in model studies. We investigated the effects of exopolysaccharides (EPSs) in cell-to-cell and cell-to-surface…

It is a controversial issue in education that students perform dissection experiments. Different opinions have been noted in studies on this subject, and various applications are recommended according to education levels. This case study aimed to determine pre-service science teachers' views of hands-on dissection and virtual dissection. The study…