In this research, it is aimed to determine the level that the organizational cultures of the employees working in sport facilities are explained by their job satisfaction and demographic characteristics. 162 employees participated in the research carried out in the relational screening model. The data of the study was collected through "Organizational Culture Scale" and "Job Satisfaction Scale". Hierarchical regression analysis was performed in the analysis of the data. As a result of the analysis, the innovative, bureaucratic and community cultures of the employees were determined to be meaningfully predicted only by job satisfaction. Besides, while the competitor cultures of the employees were significantly explained by job satisfaction, seniority, marital status and gender variables; their organizational cultures were found to be significantly explained by job satisfaction, seniority and marital status variables.