We report two visual-world eye-tracking experiments that investigated the effects of subjecthood, pronominalisation, and contrastive focus on the interpretation of pronouns in subsequent discourse. By probing the effects of these factors on real-time pronoun interpretation, we aim to contribute to our understanding of how topicality-related factors (subjecthood, givenness) interact with contrastive focus effects, and to investigate whether the seemingly mixed results obtained in prior work on topicality and focusing could be related to effects of subjecthood. Our results indicate that structural and semantic prominence (specifically, agentive subjects) influence pronoun interpretation even when separated from information-structural notions, and thus need to be taken into account when investigating topicality and focusing. We discuss how our results allow us to reconcile the distinct findings of prior studies. More generally, this research contributes to our understanding of how the language comprehension system integrates different kinds of information during real-time reference resolution. (Contains 3 tables, 5 figures and 2 footnotes.)