The purposes of this study were to examine the factorial validity of the six-item Intragroup Conflict Scale (ICS) in athletic trainer (AT) and coach populations and to examine measurement invariance by gender and profession. A total of 195 ATs and 615 head or assistant coaches working at secondary schools or National Collegiate Athletic Association (NCAA) institutions completed the ICS. Data were analyzed using confirmatory factor analysis procedures in LISREL 10 (Student Edition). Results supported the reliability and validity of the two-factor ICS model among ATs and coaches. Strict measurement invariance was observed when comparing male to female AT responses. Strong measurement invariance was observed when comparing male to female coach responses, as well as AT to coach responses. These data not only support the reliability and validity of the ICS for use in AT and coach populations but also support direct comparisons of mean scores across gender or profession.