Biology is an important subject in curriculum. It helps to develop the scientific attitude, scientific temper, logical reasoning, scientific literacy, awareness of environmental issues, and respect about surrounding life among students. Biology textbook content is an authentic source of information for teachers & students. According to the National Focus Group has made important consideration about higher secondary biology curriculum that, scientific concepts should be within reach of the learner and also content components i.e. key terms, facts, concepts, principles, illustrations etc. introduced and delivered in the classroom by meaningfully and simplified manner. The biology teachers play an important role to transfer biology content knowledge to the students. If the biology teachers already perceived some misconceptions or alternative conceptions regarding the biology concepts, it may transform as it is in their students. It will be adversely affected the conceptual understanding of the students. In the present paper researchers has made an attempt to study the perception about Biological concepts among higher secondary teachers. The content analysis method and descriptive survey method was adopted for the study. The data collected from higher secondary biology teachers (N=50) with the help of researcher made questionnaire and analyzed with the descriptive statistics. It was found that most of the higher secondary biology teachers are unaware about the biological concepts in textbook. Most of the teachers are unable to differentiate between biological terms, facts, attributes and concepts. The present paper will be helpful to know more about the present status of perception of higher secondary biology teachers and the content analysis.