Teacher education in Indonesia aims to prepare pre-service teachers in becoming professional teaching personnel. Two important knowledge need to be acquired are content knowledge (CK) and pedagogical content knowledge (PCK). However, it is still a challenge to measure the pre-service mathematics teachers' CK and PCK. Therefore, the first aim of the present study is to develop and validate a test instrument to measure CK and PCK of pre-service mathematics teachers on high school level mathematics. Second, the present study aims to measure the CK and PCK of pre-service mathematics teachers by using the developed instrument. The present study employed developmental research method and used students' empirical data in investigating the validity, objectivity, and reliability of the instrument. The results of the study and its implications for future research and practice then will be presented in the paper. [Paper presented at the South East Asia Design Research International Conference (7th, 2019.)]