Studying "Caenorhabditis elegans" ("C. elegans") live cultures provides excellent opportunities for authentic inquiry in a high school anatomy and physiology or other biology lab course. Using a digital dissection microscope, a student can photograph the organism during various stages of development and study and analyze the images. In this article, the authors describe a successful activity using a digital dissection microscope to study live cultures of "C. elegans." They adapted several laboratory protocols that address the National Science Education Standards (NRC 1996); encourage the use of inquiry; and intertwine genetics, modern molecular biology, anatomy, and physiology in one unit. Students design their own experiments and participate in inquiry-based lessons. Incorporating digital microscopes in the anatomy and physiology laboratory has increased students' ability to compare and contrast stages of development and study the behavior of "C. elegans" in greater depth. "C. elegans" is ideal to use in high school laboratories because of the organism's short life cycles, small size, and inexpensive maintenance materials. In addition, students acquired significant laboratory skills when conducting these activities. Students learned how to use microscopes, to distinguish the sexes, to characterize wild-type and mutant worms, to observe behaviors in different environments such as those in which there are different chemical substances, and to maintain cultures of C. elegans. (Contains 3 figures.)