Background: Alcohol consumption is common among U.S. adults. The purpose of this study was to describe the source and content of the most widely viewed YouTube videos associated with alcohol consumption. Methods: Videos were filtered by number of views and the source of the video upload was recorded. Video characteristics and differences in video content were then assessed. Results: All together, the videos in this sample were viewed 21,481,530 times. Consumers uploaded the majority of videos found in this sample. Most videos mentioned the risks of drinking (88.1%, 95% CI: 80.3, 95.9%) and advised viewers to quit drinking (86.9%, 95% CI: 82.5, 91.3%). More than half of the videos uploaded by television and internet sources (73.7%, 95% CI: 62.6%, 84.8%) or medical professionals (69.2%, 95% CI: 53.1%, 85.3%) referred to previous studies or research, but less than half of the videos uploaded from other sources did so. Conclusion: The risks associated with alcohol consumption and limits on drinking were frequently mentioned, with consumers frequently encouraged to quit drinking. Future work is needed to broaden our understanding of alcohol content across the site and over time in behavioral development.