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Stephanie A. Carr; Stephanie L. Mathews; Justin A. Pruneski; Nikolas M. Stasulli – Journal of Microbiology & Biology Education, 2024
In this article, we describe curricular materials developed to engage undergraduate students in evolutionary thinking around antibiotic resistance using the MEGA-plate experiment (Microbial Evolution and Growth Arena). This elegant and visual experiment, developed by the Kishony Lab, shows the development of antibiotic resistance on the timescale…
Descriptors: Biochemistry, Science Instruction, Laboratory Experiments, Drug Therapy
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R. A. Paggi; M. C. Ferrari; M. Cerletti; M. I. Giménez; T. S. Schwarz; A. Marchfelder; R. E. De Castro – Biochemistry and Molecular Biology Education, 2025
Perturbation of gene expression using RNA interference (RNAi) or CRISPR interference (CRISPRi) is a useful strategy to explore the function of essential genes. In the archaeon "Haloferax volcanii," the CRISPR-Cas system has been adapted as a CRISPRi tool to silence the expression of specific genes. We developed a laboratory class (LC) to…
Descriptors: Undergraduate Students, Laboratory Experiments, Science Instruction, Biochemistry
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Vagula, Mary; Liu, He – HAPS Educator, 2018
Students find it hard to understand acid-base homeostasis and the mechanisms involved in compensating for acid-base imbalances, including the role of the lungs and kidneys in this process. We have developed a laboratory activity based on collaborative problem-based learning and human patient simulators (HPSs) to teach this topic. Students (n=50)…
Descriptors: Physiology, Science Instruction, Anatomy, Heart Disorders
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Boyd-Kimball, Debra; Miller, Keith R. – Journal of Chemical Education, 2018
Laboratory courses are often designed using step-by-step protocols which encourage students to conduct experiments without thinking about what they are doing or why they are doing it. Such course design limits the growth of our students as scientists and can make it more difficult for a student to transition to the expectations of a research…
Descriptors: Science Laboratories, Science Instruction, College Science, Undergraduate Study
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Guerra, Nelson Pérez – Journal of Chemical Education, 2017
A laboratory experiment in which students study the kinetics of the Viscozyme-L-catalyzed hydrolysis of cellulose and starch comparatively was designed for an upper-division biochemistry laboratory. The main objective of this experiment was to provide an opportunity to perform enhanced enzyme kinetics data analysis using appropriate informatics…
Descriptors: Undergraduate Study, Biochemistry, Kinetics, Laboratory Experiments
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Taipa, M. A^ngela; Azevedo, Ana M.; Grilo, Anto´nio L.; Couto, Pedro T.; Ferreira, Filipe A. G.; Fortuna, Ana R. M.; Pinto, Ine^s F.; Santos, Rafael M.; Santos, Susana B. – Journal of Chemical Education, 2015
An integrative laboratory study addressing fundamentals of enzyme catalysis and their application to reactors operation and modeling is presented. Invertase, a ß-fructofuranosidase that catalyses the hydrolysis of sucrose, is used as the model enzyme at optimal conditions (pH 4.5 and 45 °C). The experimental work involves 3 h of laboratory time…
Descriptors: Cooperative Learning, Science Experiments, College Science, Undergraduate Study
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Evans, Hedeel Guy; Heyl, Deborah L.; Liggit, Peggy – Journal of Chemical Education, 2016
This biochemistry laboratory course was designed to provide significant learning experiences to expose students to different ways of succeeding as scientists in academia and foster development and improvement of their potential and competency as the next generation of investigators. To meet these goals, the laboratory course employs three…
Descriptors: Biochemistry, Science Instruction, Course Evaluation, College Faculty
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Faraldos, Juan A.; Giner, Jos-Luis; Smith, David H.; Wilson, Mark; Ronhovde, Kyla; Wilson, Erin; Clevette, David; Holmes, Andrea E.; Rouhier, Kerry – Journal of Chemical Education, 2011
This organic laboratory experiment introduces students to stereoselective enzyme reactions, resolution of enantiomers, and NMR analysis of diastereomers. The reaction between racemic 1-phenylethanol and vinyl acetate in hexane to form an ester is catalyzed by acylase I. The unreacted alcohol is then treated with a chiral acid and the resulting…
Descriptors: Biochemistry, Laboratory Experiments, Science Instruction, Scientific Principles
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Goeden, Terrah J.; Kurtz, Martha J.; Quitadamo, Ian J.; Thomas, Carin – Journal of Chemical Education, 2015
In the Community-Based Inquiry (CBI) instructional method, cooperative student groups complete case study activities based on scientific literature and conduct their own laboratory investigations that address authentic community needs. This study compared critical thinking and content knowledge outcomes between traditional Introduction to…
Descriptors: Science Instruction, Inquiry, Cooperative Learning, Case Studies
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Kuhn, Misty L.; Figueroa, Carlos M.; Aleanzi, Mabel; Olsen, Kenneth W.; Iglesias, Alberto A.; Ballicora, Miguel A. – Biochemistry and Molecular Biology Education, 2010
Higher education institutions and scientific funding agencies are emphasizing international projects that involve the integration and synergy between research groups, particularly if different disciplines are involved. Students with an education that reflects these trends will have more tools to succeed in the future, but it is challenging to…
Descriptors: Foreign Countries, Interdisciplinary Approach, Cooperative Learning, Graduate Students
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Stilts, Corey E.; Fisher, Roxanne – Journal of Chemical Education, 2007
Bioassays are commonly used to test the biological activity of chemicals and other exercises are presented in which students synthesize plant hormones. Lab exercise is conducted using commercially available auxins and auxin regulating compounds.
Descriptors: Science Activities, Organic Chemistry, Laboratory Experiments, Science Experiments