Discusses selected bioinorganic aspects of carbonic anhydrase and describes experiments that will reinforce the students' understanding of the presence and essential role that metal ions have in some biological systems. (SL)

Describes an experiment which can be used to provide an introduction to study of reaction mechanisms and training in use of simple analytical and instrumental techniques. (PS)

An experimental procedure is described for studying oxygen uptake by a transition metal. This procedure is designed for use with undergraduates and may be used in organic or biochemistry laboratories. Diagrams of the apparatus are included. (MR)

The experiment described here involves simultaneous monitoring of pH and NMR chemical shifts during an aqueous titration of alpha- and beta-alanine. This experiment is designed for use in an undergraduate biochemistry course. (MR)

Two experiments are described and appropriate discussion is given to illustrate the use of substoichiometry and saturation analysis techniques with undergraduates. The first experiment is the determination of silver content in photographic film. The second is the estimation of a hormone concentration using saturation analysis and a commercially…

Experimental procedures for studying enzyme activity using a Spectronic 20 spectrophotometer are described. The experiments demonstrate the effect of pH, temperature, and inhibitors on enzyme activity and allow the determination of Km, Vmax, and Kcat. These procedures are designed for teaching large lower-level biochemistry classes. (MR)