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Direct linkAlison Bates; Kathryn M. Williams; Ann E. Hagerman – Biochemistry and Molecular Biology Education, 2025
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…
Descriptors: Undergraduate Students, Science Instruction, Laboratory Experiments, Kinetics
Andrew J. Tonsager; Laurie A. Stargell – Biochemistry and Molecular Biology Education, 2025
CRISPR-Cas9 technology is an established, powerful tool for genome editing through the ability to target specific DNA sequences of interest for introduction of desired genetic modifications. CRISPR-Cas9 is utilized for a variety of purposes, ranging from a research molecular biology tool to treatment for human diseases. Due to its prominence…
Descriptors: Undergraduate Students, Student Research, Scientific Research, Genetics
Maurye, Praveen; Basu, Arpita; Jana, Chayna; Devi, Manoharmayum Shaya; Kumari, Kavita; Naskar, Malay – Biochemistry and Molecular Biology Education, 2022
Gel electrophoresis (GE) is the most preferred and adapted technique for the separation and identification of biological molecules like proteins/peptides and nucleic acids from diverse types of organisms. All over the world, researchers, educators, and students aspiring to work in biochemistry and molecular biology disciplines use the…
Descriptors: High School Students, Biochemistry, Molecular Biology, Laboratory Procedures
Candela, Maria Fernanda; Arenas, Nelson E.; Caicedo, Obradith; Malagon, Andres – Journal of Chemical Education, 2021
The inhibition of porcine pancreatic lipase by the drug orlistat was studied through a series of activities that combine experimental and computational procedures. The enzymatic activity of the lipase was determined by measuring enzyme kinetics and calculating parameters such as K[subscript m] and V[subscript max] from experimental data. Assays of…
Descriptors: Biochemistry, Drug Therapy, Kinetics, Measurement
Nicola´s Andre´s Saffioti; Carla Angelani; Jose´ Mari´a Delfino; Silvia Ferna´ndez Villamil; Gabriela Elena Go´mez; Irene Mangialavori; Mari´a Florencia Pignataro; Pablo Julio Schwarzbaum – Journal of Chemical Education, 2023
Metabolic Control Analysis (MCA) is a systems biology approach that measures the control of a metabolic step on the overall flux of a metabolic pathway. In undergraduate courses in biochemistry, learning of MCA can be encouraged by experimental activities demonstrating practical applications of the MCA theory. In this work, we present a laboratory…
Descriptors: Biochemistry, Metabolism, College Science, Undergraduate Students
Thaveesangsakulthai, Isaya; Kulsing, Chadin – Journal of Chemical Education, 2021
Optimization of experimental conditions in chromatography is recognized as a wear-and-tear process involving hundreds of experiments in order to maximize the number of separated compounds for each sample. The critical thinking skills and practices entailed cannot be achieved with students in a conventional lecture class. This report provides a…
Descriptors: Spreadsheets, Simulation, Biochemistry, Laboratory Procedures
Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna – Biochemistry and Molecular Biology Education, 2021
The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In…
Descriptors: Biochemistry, Cytology, Metabolism, Science Laboratories
Vuppala, Laxmi Sinduri; Vazquez, Timothy; Nguyen, Ding; Knowlton, Maverick; Smith, Emily; Evan-Nguyen, Theresa – Journal of Chemical Education, 2020
Exposure to real-life applications of quantitative analysis techniques based on two gas chromatography (GC) experiments was provided to a large cohort of sophomore-level students. In one experiment, students assessed blood alcohol content by internal standardization using a homemade headspace system similar to methods implemented in forensic…
Descriptors: Undergraduate Students, Laboratory Experiments, Science Experiments, Biochemistry
Download full textMuhammad Saefi; Widi Cahya Adi; Amining Rahmasiwi; Hidayati Maghfiroh; M. Eval Setiawan; Miza Nina Adlini; Syarif Rizalia – Journal of Biological Education Indonesia (Jurnal Pendidikan Biologi Indonesia), 2023
Laboratory course activities often focus on "cookbook style," so their activities have not yet integrated with scientific thinking as an essential aspect. In this research, the laboratory experience of microbial analysis techniques was redesigned using alternative inquiry to involve students in isolating and characterising yeast to make…
Descriptors: Laboratory Experiments, Teaching Methods, Food, Cooking Instruction
Angelani, Carla R.; Carabias, Pablo; Cruz, Karen M.; Delfino, José M.; Sautu, Marilina; Espelt, María V.; Ferreira-Gomes, Mariela S.; Gómez, Gabriela E.; Mangialavori, Irene C.; Manzi, Malena; Pignataro, María F.; Saffioti, Nicolás A.; Salvatierra Fréchou, Damiana M.; Santos, Javier; Schwarzbaum, Pablo J. – Biochemistry and Molecular Biology Education, 2018
Metabolic control analysis (MCA) is a promising approach in biochemistry aimed at understanding processes in a quantitative fashion. Here the contribution of enzymes and transporters to the control of a given pathway flux and metabolite concentrations is determined and expressed quantitatively by means of numerical coefficients. Metabolic flux can…
Descriptors: Metabolism, Biochemistry, Cytology, Laboratory Procedures
Odunuga, Odutayo O.; Cheatwood, Nicholas Y.; Mullins, John A.; Nguyen, Samantha K.; Fry, Darrell R.; Harris, Michele R. – Journal of Chemical Education, 2021
This paper describes an undergraduate biochemistry laboratory module consisting of a set of experiments designed around a purification scheme for bovine serum albumin (BSA). Students purify BSA from cow plasma by a combination of salt and acetone precipitation, equilibrium dialysis, ion exchange, and size exclusion chromatography. Students use the…
Descriptors: Undergraduate Students, College Science, Science Instruction, Instructional Design
Ortiz Lechuga, Eugenia Guadalupe; Tovar Herrera, Omar Eduardo; Arévalo Niño, Katiushka – Biochemistry and Molecular Biology Education, 2019
In this work, we present the results of an undergrad student from the perspective of its first approach as a principal researcher in a project. In order to gain practical experience, one of the options for students that have interest in pursuing a postgraduate program corresponds to a research stay in a laboratory of their selected field…
Descriptors: Undergraduate Students, College Science, Biotechnology, Biochemistry
Nagma Zerin – Chemical Engineering Education, 2024
Project-Enhanced learning is an excellent way to facilitate student-centered learning along with traditional lecture-based learning. In this Class and Home problem, an example of Project-Enhanced learning is provided that can be used in the Mass and Energy Balances (MEB) course. The students solve this problem as part of a group while receiving…
Descriptors: Student Projects, Active Learning, Student Centered Learning, Teaching Methods
Marsiglia, William M.; Qamra, Rohini; Jackson, Kimberly M.; Traaseth, Nathaniel J. – Journal of Chemical Education, 2020
The design of undergraduate laboratory courses that provide meaningful research-based experiences enhances undergraduate curricula and prepares future graduate students for research careers. In this article, a course-based undergraduate research experience (CURE) laboratory module was designed for upper-division undergraduate biochemistry and…
Descriptors: Biochemistry, Science Instruction, College Science, Student Research
Quilis, Inma; Igual, J. Carlos – Biochemistry and Molecular Biology Education, 2018
This article describes a laboratory exercise designed for undergraduate students in the subject of "Regulation of cell proliferation" which allows the students to carry out a research experiment in an important field such as cell cycle control, and to be introduced to a widely used technique in molecular biology laboratories such as the…
Descriptors: Cytology, Biochemistry, Laboratory Experiments, Molecular Biology
