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The Real Reason Why ATP Hydrolysis Is Spontaneous at pH > 7: It's (Mostly) the Proton Concentration!
Peer reviewed
Direct linkSilverstein, Todd P. – Biochemistry and Molecular Biology Education, 2023
Common wisdom holds that ATP hydrolysis is spontaneous because of the weakness of its phosphoanhydride bonds, electrostatic repulsion within the polyanionic ATP[superscript 4]-molecule, and resonance stabilization of the inorganic phosphate and ADP products. By examining the pH-dependence of the hydrolysis Gibbs free energy, we show that in fact,…
Descriptors: Biochemistry, Molecular Biology, Science Education, Scientific Concepts
Murphy Angelo; Yash Bhargava; Scott Takeo Aoki – Biochemistry and Molecular Biology Education, 2024
The complexity of RNA cannot be fully expressed with the canonical A, C, G, and U alphabet. To date, over 170 distinct chemical modifications to RNA have been discovered in living systems. RNA modifications can profoundly impact the cellular outcomes of messenger RNAs (mRNAs), transfer and ribosomal RNAs, and noncoding RNAs. Additionally, aberrant…
Descriptors: Genetics, Biochemistry, Molecular Biology, College Science
Naoto Shirasu; Shin'ichiro Yasunaga – Biochemistry and Molecular Biology Education, 2024
Here, we propose a laboratory exercise to quickly determine single nucleotide polymorphisms (SNPs) in human "alcohol dehydrogenase 1B (ADH1B)" and "aldehyde dehydrogenase 2 (ALDH2)" genes involved in alcohol metabolism. In this exercise, two different genotyping methods based on polymerase chain reaction (PCR), namely…
Descriptors: Genetics, Biochemistry, Students, Science Instruction
Tiantian Cheng; Lingzhen Cao; Meng Zou – American Biology Teacher, 2024
The central dogma of molecular biology describes the transfer of genetic information from nucleic acids to proteins and stipulates that the system cannot work in the reverse direction. As a fundamental principle in biology, the dogma is as influential as it is controversial. Some commentators have debated the central dogma's empirical accuracy…
Descriptors: Biology, Genetics, Science Instruction, Physiology
Download full textJosep J. Centelles; Estefania Moreno; Pedro R. de Atauri – International Society for Technology, Education, and Science, 2023
Gamification is a very useful technique, that has been fully increasing in the last years. During the COVID-19 pandemic, our innovation teaching group sent to our students several games, that were used by them for self-studying. Considering that many numerical problems can be proposed in Biochemistry, rather than numerical games (sudoku, adding…
Descriptors: Gamification, Biochemistry, Vocabulary, Educational Games
Craig, Paul A.; Nash, Jessica A.; Crawford, T. Daniel – Biochemistry and Molecular Biology Education, 2022
A programming workshop has been developed for biochemists and molecular biologists to introduce them to the power and flexibility of solving problems with Python. The workshop is designed to move users beyond a "plug-and-play" approach that is based on spreadsheets and web applications in their teaching and research to writing scripts to…
Descriptors: Programming Languages, Biochemistry, Molecular Biology, Data Analysis
Ludmila Tyler; Peter J. Kennelly; Shelly Engelman; Kirsten F. Block; Jennifer C. Bobenko; Jaclyn Catalano; Jesica A. Jones; Margaret I. Kanipes-Spinks; Yang Mooi Lim; Jennifer Loertscher; Tejiri Olafimihan; Hailey Reiss; Territa L. Upchurch-Poole; Yufeng Wei; Kimberly J. Linenberger Cortes; Victoria Del Gaizo Moore; Daniel R. Dries – Biochemistry and Molecular Biology Education, 2024
We present as a case study the evolution of a series of participant-centered workshops designed to meet a need in the life sciences education community--the incorporation of best practices in the assessment of student learning. Initially, the ICABL (Inclusive Community for the Assessment of Biochemistry and Molecular Biology/BMB Learning) project…
Descriptors: Science Education, Science Teachers, Teacher Workshops, Biological Sciences
Singh, Anoushka; Miller, Ryan C.; Archuleta, Stephen R.; Kugel, Jennifer F. – Biochemistry and Molecular Biology Education, 2023
Transcription is the critical first step in expressing a gene, during which an RNA polymerase (RNAP) synthesizes an RNA copy of one strand of the DNA that encodes a gene. Here we describe a laboratory experiment that uses a single assay to probe two important steps in transcription: (1) RNAP binding to DNA, and (2) the transcriptional activity of…
Descriptors: Molecular Biology, Science Instruction, Genetics, Teaching Methods
Risanti Dhaniaputri; Hadi Suwono; Betty Lukiati – Journal of Biological Education Indonesia (Jurnal Pendidikan Biologi Indonesia), 2024
Plant metabolite compounds have been applied on plant cellular metabolism, produces organic and inorganic compounds, primary and secondary bioactive molecules, such as glucose, amino acids, fat acids, alkaloids, flavonoids, and terpenoids. Undergraduate biology students learn about the plant terpenoid assisted by bioinformatics to store, manage,…
Descriptors: Foreign Countries, Science Instruction, Biology, Plants (Botany)
Maurye, Praveen; Basu, Arpita; Jana, Chayna; Devi, Manoharmayum Shaya; Kumari, Kavita; Naskar, Malay – Biochemistry and Molecular Biology Education, 2022
Gel electrophoresis (GE) is the most preferred and adapted technique for the separation and identification of biological molecules like proteins/peptides and nucleic acids from diverse types of organisms. All over the world, researchers, educators, and students aspiring to work in biochemistry and molecular biology disciplines use the…
Descriptors: High School Students, Biochemistry, Molecular Biology, Laboratory Procedures
Meftahi, Gholam Hossein; Bahari, Zahra; Zarei Mahmoudabadi, Ali; Iman, Maryam; Jangravi, Zohreh – Biochemistry and Molecular Biology Education, 2021
Western blot (WB) or immunoblot is a workhorse method. It is commonly used by biologists for study of different aspects of protein biomolecules. In addition, it has been widely used in disease diagnosis. Despite some limitations such as long time, different applications of WB have not been limited. In the present review, we have summarized…
Descriptors: Biochemistry, Molecular Biology, Clinical Diagnosis, Literature Reviews
María Florencia Mosquillo; Gonzalo Scalese; Felipe Castro; Leticia Pérez-Díaz – Natural Sciences Education, 2024
An effective, practical approach for incorporating molecular biology tools to biology and biochemistry students is described. For 6 weeks, students are exposed to bacterial transformation, plasmid DNA purification and analysis, parasite transfection, ectopic expression of fluorescent proteins, fluorescence microscopy, and flow cytometry analysis.…
Descriptors: Active Learning, Student Projects, Science Education, Molecular Biology
R. A. Paggi; M. C. Ferrari; M. Cerletti; M. I. Giménez; T. S. Schwarz; A. Marchfelder; R. E. De Castro – Biochemistry and Molecular Biology Education, 2025
Perturbation of gene expression using RNA interference (RNAi) or CRISPR interference (CRISPRi) is a useful strategy to explore the function of essential genes. In the archaeon "Haloferax volcanii," the CRISPR-Cas system has been adapted as a CRISPRi tool to silence the expression of specific genes. We developed a laboratory class (LC) to…
Descriptors: Undergraduate Students, Laboratory Experiments, Science Instruction, Biochemistry
Jakubowski, Henry V.; Bock, Nicholas; Busta, Lucas; Pearce, Matthew; Roston, Rebecca L.; Shomo, Zachery D.; Terrell, Cassidy R. – Biochemistry and Molecular Biology Education, 2021
Our climate is changing due to anthropogenic emissions of greenhouse gases from the production and use of fossil fuels. Present atmospheric levels of CO[subscript 2] were last seen 3 million years ago, when planetary temperature sustained high Arctic camels. As scientists and educators, we should feel a professional responsibility to discuss major…
Descriptors: Science Instruction, Climate, Change, Ecology
Didem Vardar-Ulu; Saif Eldeen Ragab; Swati Agrawal; Shuchismita Dutta – Journal of Microbiology & Biology Education, 2024
Molecular case studies (MCSs) are open educational resources that use a storytelling approach to engage students in biomolecular structure-function explorations, at the interface of biology and chemistry. Although MCSs are developed for a particular target audience with specific learning goals, they are suitable for implementation in multiple…
Descriptors: Computer Simulation, Educational Technology, Molecular Biology, Undergraduate Students
