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Lewis Rolband; Varsha Godakhindi; Juan L. Vivero-Escoto; Kirill A. Afonin – Journal of Chemical Education, 2023
Investigating and understanding novel antibacterial agents is a necessary task as there is a constant increase in the number of multidrug-resistant bacterial species. The use of nanotechnology to combat drug-resistant bacteria is an important research area. The laboratory experiment described herein demonstrates that changes in the nanostructure…
Descriptors: Undergraduate Students, Graduate Students, Microbiology, Biochemistry
Yan, Jingmin; Chen, Jiangao; Mao, Xuhu; Li, Qian – Biochemistry and Molecular Biology Education, 2023
As a universal and extensively adopted technique, enzyme-linked immunosorbent assay (ELISA) can be used to detect and quantify small molecules in many applications both clinical and analytical. However, generally, students experiment mechanically using commercial ELISA kits according to the instructions and eventually produce a standard curve to…
Descriptors: Undergraduate Students, Science Instruction, Biotechnology, Biochemistry
Crane, Julia; Page, Shallee T. – Biochemistry and Molecular Biology Education, 2022
We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published…
Descriptors: Undergraduate Students, College Science, Science Instruction, Scientific Research
Plasencia, Javier – Biochemistry and Molecular Biology Education, 2023
An undergraduate online course on Applied Biochemistry and Molecular Biology was developed through different formats of case study that included lecture, class discussion, small-groups discussion, and individual work. Cases covering health, biotechnology, agriculture, and other issues were developed or adapted from the literature to reach the…
Descriptors: Online Courses, Biochemistry, Molecular Biology, Undergraduate Students
Rosini, Elena; Pollegioni, Loredano – Biochemistry and Molecular Biology Education, 2019
Protein engineering represents a modern approach to generate novel proteins for the different fields of biotechnology. Here, we report about an 8-day laboratory activity in which students generate enzyme variants to degrade the herbicide glyphosate. The students conduct a true research experiment in an important field (bioremediation by novel,…
Descriptors: Science Laboratories, Biotechnology, Engineering, Biochemistry
Santos, Tiago; Pereira, Patrícia; Queiroz, João A.; Cruz, Carla; Sousa, Fani – Biochemistry and Molecular Biology Education, 2019
This laboratory experiment describes the production and purification of plasmid DNA for undergraduate biochemistry and biotechnology courses. This experiment performed in a one-week period includes the protocols for plasmid pVAX1-LacZ production in "Escherichia coli" DH5[alpha] cells and subsequent purification of supercoiled pVAX1-LacZ.…
Descriptors: Biotechnology, Biochemistry, Laboratory Experiments, Undergraduate Students
Rutter, Charles; Pancorbo, Jennifer – Chemical Engineering Education, 2020
Fermentation is responsible for the production of myriad products across a variety of industrial sectors. In particular, the biomanufacturing industry requires a labor force proficient in fermentation and its associated technologies to drive the production of recombinant protein therapeutics. This paper describes the development of a course that…
Descriptors: Biotechnology, Manufacturing Industry, Biochemistry, Scientific Concepts
Iametti, Stefania; Barbiroli, Alberto; Marengo, Mauro; Bonomi, Francesco – Biochemistry and Molecular Biology Education, 2019
Biochemistry has always been a mandatory topic within BS courses aimed at food science students at the University of Milan, namely: Food Science & Technology and Catering Sciences. Addressing biochemistry topics in this peculiar setting requires: (i) specific focus on topics that are seldom considered in courses offered in bio-medical…
Descriptors: Biochemistry, Science Instruction, College Students, Food
Lindsay, Andrew J. – Journal of Chemical Education, 2019
A semester-long, project-based series of laboratory exercises is described that provides practical experience of the upstream and downstream processes involved in monoclonal antibody production. This 6 week project is aimed at final year undergraduate students undertaking primary degrees in biotechnology, biochemistry, or related disciplines.…
Descriptors: Biotechnology, Undergraduate Students, Student Projects, Active Learning
Sajja, Sameer; Chandler, Morgan; Striplin, Caryn D.; Afonin, Kirill A. – Journal of Chemical Education, 2018
With the advancement of ribonucleic acid (RNA) research in the field of biochemistry, ensuring that undergraduate students have both the theoretical and practical knowledge of current, cutting-edge experimental techniques is of marked importance. Many current biochemistry experiments demonstrate various basic molecular biological techniques…
Descriptors: Science Instruction, Biochemistry, Biotechnology, Spectroscopy
Torres, Néstor; Santos, Guido – Higher Education Pedagogies, 2017
Enzyme kinetics is an essential part of biochemistry programs, which have been gaining importance in recent years for their applications in biotechnology and biomedicine. The teaching and learning of these issues has been traditionally hampered by difficulties that stem mainly from the dynamic and mathematical nature of the topic and the…
Descriptors: Simulation, Kinetics, Problem Solving, Biochemistry
Brunauer, Linda S. – Journal of Chemical Education, 2016
A multiweek protein purification suite, suitable for upper-division biochemistry or biotechnology undergraduate students, is described. Students work in small teams to isolate the enzyme lactate dehydrogenase (LDH) from a nontraditional tissue source, mammalian blood, using a sequence of three column chromatographic procedures: ion-exchange, size…
Descriptors: Biochemistry, Biotechnology, Undergraduate Students, Science Instruction
Brunauer, Linda S.; Caslavka, Katelyn E.; Van Groningen, Karinne – Journal of Chemical Education, 2014
A multiday laboratory exercise is described that is suitable for first-year undergraduate chemistry, biochemistry, or biotechnology students. Students gain experience in performing chromatographic separations of biomolecules, in both a column and thin layer chromatography (TLC) format. Students chromatographically separate amino acids (AA) in an…
Descriptors: Science Experiments, Laboratory Experiments, College Science, Undergraduate Students
Greenler, John; Nye, Leith; Tangen, Travis – Science Teacher, 2014
Production of liquid fuels such as ethanol from fibrous plant biomass could potentially be a significant sustainable component of the U.S. energy portfolio. Engineers and scientists are actively researching this area, and high school students can engage in this contemporary inquiry process by experimenting with different types of biomass, varying…
Descriptors: Science Instruction, Fuels, Energy, High School Students
Wallert, Mark A.; Provost, Joseph J. – Biochemistry and Molecular Biology Education, 2014
To enhance the preparedness of graduates from the Biochemistry and Biotechnology (BCBT) Major at Minnesota State University Moorhead for employment in the bioscience industry we have developed a new Industry certificate program. The BCBT Industry Certificate was developed to address specific skill sets that local, regional, and national industry…
Descriptors: Biochemistry, Biotechnology, Undergraduate Students, Career Readiness