In response to the shifting modalities of laboratory-based education, particularly amidst the rise of virtual and at-home instruction, we introduce a novel hybrid laboratory module for undergraduate biochemistry and biology courses. Central to this innovative approach is the use of sourdough as a versatile and accessible model organism, enabling a…

Although many laboratory experiments are available to illustrate spectrophotometric or fluorometric methods, few of them introduce the use of luminometry to students. Bioluminescence, a subtype of chemiluminescence, is produced when an enzyme-catalyzed chemical reaction gives rise to light emission. Despite the advantages of bioluminescent…

Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to…

"Sinorhizobium meliloti" is a bacterium of great agroeconomic importance because of its ability to fix atmospheric nitrogen in symbiotic association with alfalfa ("Medicago sativa") roots, and it is often used in model studies. We investigated the effects of exopolysaccharides (EPSs) in cell-to-cell and cell-to-surface…

The bacterial cell wall, a structural unit of peptidoglycan polymer comprised of glycan strands consisting of a repeating disaccharide motif [N-acetylglucosamine (NAG) and N-acetylmuramylpentapeptide (NAM pentapeptide)], encases bacteria and provides structural integrity and protection. Lysozymes are enzymes that break down the bacterial cell wall…

Biochemistry courses in the Department of Molecular Biology at the National University of Río Cuarto, Argentina, are designed for undergraduate students in biology, microbiology, chemistry, agronomy, and veterinary medicine. Microbiology students typically have previous coursework in general, analytical, and organic chemistry. Programmed sequences…

This case has the student actively investigate the regulation of expression of a novel bacterial gene in the context of attempts to solve a real world problem, clean up of the April 2010 Deep Water Horizon oil spill in the Gulf of Mexico. Although the case is fictitious, it is based on factual gene regulatory characteristics of oil-degrading…

The yeast "Saccharomyces cerevisiae" has been used by human beings since ancient times for its ability to convert sugar to alcohol. Continual exposure to glucose in the natural environment for innumerable generations has probably enabled "S. cerevisiae" to grow in fermentative mode on sugars by switching off the genes responsible for respiration…

Criticizes introductory biology texts for merely describing living things in terms of what they can do as whole organisms and neglecting to consider the nature of life on a micro-scale. Presents possibilities for including such discussions in an introductory biology course. Contains 14 references. (WRM)

Presents a novel idea for teaching a scientific concept using a poem that describes the major perspectives on the origins of living systems as the medium of instruction. Addresses major concepts such as chemical evolution, DNA vs. RNA, protocell formation, coacervates, panspermia, and special creation. (Author/CCM)