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Peer reviewedHurlbut, Jeffrey A.; And Others – Journal of Chemical Education, 1977
Experimental procedures for studying enzyme activity using a Spectronic 20 spectrophotometer are described. The experiments demonstrate the effect of pH, temperature, and inhibitors on enzyme activity and allow the determination of Km, Vmax, and Kcat. These procedures are designed for teaching large lower-level biochemistry classes. (MR)
Descriptors: Biochemistry, Chemistry, College Science, Higher Education
