Student ethnicity is one of the internal factors that influence student learning. Bacteriology is a course that examines prokaryotic unicellular living things and is practiced in laboratories that need to implement projects. The aim of this research is to know the distribution of students based on their ethnicity. The research sample was students…

This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel­(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…

Enzymes provide attractive targets for drug development. Quantitative analysis of enzyme reactions can lead to rational drug discovery. For undergraduates majoring in biochemistry or pharmacy and engaged in drug discovery-related work, it is essential to acquire a comprehensive understanding of enzyme kinetics, including experimental methods and…

Failing test scores in reading proficiency have been a blemish on our nation's report card for decades as scores have remained static despite the readily-available information on language and literacy attainment, and the employment of research-based strategies in classrooms. While we continue our quest to efficiently bolster reading skills, a less…

Mass spectrometry is essential for large-scale protein identification in proteomics. By database searching of mass spectrometry data, it is possible to identify proteins without the need to interpret MS/MS spectra (de novo sequencing), which is time-consuming and not feasible as a systematic tool in proteomic analysis. Many authors have reported…

We are living in the Big Data era, and yet we may have serious troubles when dealing with a handful of kinetic data if we are not properly instructed. The aim of this paper, related to enzyme kinetics, is to illustrate how to determine the K[subscript m] and V[subscript max] of a michaelian enzyme avoiding the pitfalls in which we often fall. To…

While we have been looking for alternative educational materials for our undergraduate students, we found ourselves in COVID-19 pandemic and had to think postgraduate education. On the first day of restrictions, our major problem was to learn and find the best communication tool. The first experiences for online meetings were boring, we were not…

Virtual labs have proven to be very versatile and valuable for providing realistic experience for students in STE (Science, Technology, and Engineering) fields. As we progress from delivery of instructional material from classroom to the Internet, there is need to develop laboratory exercises to supplement the theoretical lessons. A Web-based…

Strong evidence supports a role for protein degradation in fear memory formation. However, these data have been largely done in only male animals. Here, we found that following contextual fear conditioning, females, but not males, had increased levels of proteasome activity and K48 polyubiquitin protein targeting in the dorsal hippocampus, the…

Advances in physiology and other fields are strongly associated with a solid base knowledge of biochemistry and cell metabolism. On the other hand, the complex and abstract nature of metabolic pathways, the traditional lecture method, and other factors made the teaching-learning process of biochemistry a challenging endeavor. To overcome this, we…

Although many laboratory experiments are available to illustrate spectrophotometric or fluorometric methods, few of them introduce the use of luminometry to students. Bioluminescence, a subtype of chemiluminescence, is produced when an enzyme-catalyzed chemical reaction gives rise to light emission. Despite the advantages of bioluminescent…

Scientific knowledge is growing rapidly, particularly in biochemistry, an interdisciplinary subject in which the courses attract diverse students with varied disciplinary interests and career goals. Thus, there is a need to teach skills and concepts in biochemistry in a way that is both compelling to a variety of learners and also relevant to…

A laboratory exercise for undergraduate advanced students of enzymology and biocatalysis is presented. Since enzyme assays can be quenched or continuous, this experiment compares the performance of two quenching agents for lactase, in a continuous setup. Enzymatic activity of [beta]-galactosidase ("Aspergillus oryzae") was determined…

The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In…

While accumulating literature is demonstrating the role of the dopamine transporter (DAT) in predicting emotional-behavioural difficulties in adults of at risk populations, only few studies have focused on the possible association between the methylation status of the DAT promoter and the psychopathological risk of mothers, fathers and children in…