This study highlights the importance of incorporating modern mass spectrometry techniques into undergraduate education. By focusing on the structure-characterizing capability of MS, students are able to gain hands-on experience with cutting-edge techniques and better understand how MS can be used to solve real-world problems. The three-step lipid…

This communication describes an updated experiment of peptide synthesis, involving determination of the loading capacity of a Fmoc-protected amino functionalized resin using UV-vis spectroscopy and a subsequent synthesis of a dipeptide in order to exemplify the experimental solid-phase synthesis procedure as an alternative technique in organic…

This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel­(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…

An integrated laboratory experience using metal--organic frameworks (MOFs), a nanoporous and crystalline extended solid material composed of metal cations and organic linkers, was developed and adopted for upper-division, major-level chemistry and biochemistry students. In this laboratory, students were guided through the facile solvothermal…

This undergraduate laboratory experiment is designed for first-year general chemistry students with the goal of introducing the brilliant colors and magnetic properties of transition metal complexes within a bioinorganic/cell biology context. In the first laboratory period, a coordination complex formed from ferric chloride and maltol is…

Techniques and instrumentation that involve fluorescence are used in many careers related to biology and biochemistry. However, due to the expense of instrumentation, the theory and practice of fluorescence spectroscopy is commonly concentrated in instrumental analysis in most curricula, a class rarely required for biology and biochemistry majors.…

Eleven different laboratory experiments were designed and executed throughout a semester to provide a meaningful research experience for 23 undergraduate biochemistry majors at UTSA. The topic of the semester was based on the idea of exploring new aspects to enhance our understanding of how the human body uses cytochrome P450 enzymes to metabolize…

An upper-division undergraduate- or graduate-level laboratory experiment, challenging students to identify bovine serum albumin, equine myoglobin, bovine calmodulin, and bovine cytochrome c, uses four methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): linear positive, in-source decay, peptide…

The design of undergraduate laboratory courses that provide meaningful research-based experiences enhances undergraduate curricula and prepares future graduate students for research careers. In this article, a course-based undergraduate research experience (CURE) laboratory module was designed for upper-division undergraduate biochemistry and…

Two-dimensional nuclear magnetic resonance (2D NMR) techniques are regularly used for the determination of RNA structures. In an effort to introduce advanced level students who are generally not acquainted with or exposed to these techniques, this work, which has been designed as a practicum, utilizes a combination of 2D NMR techniques to help…

A modular experiment for upper-level undergraduate students is presented, featuring the product cycle and process evaluating the quality and quantity of biodiesel obtained from a microalgae feedstock grown under carbon-supplemented and nutrient-deprived conditions. Batch reactors are used to cultivate and harvest microalgae, which undergo…

Mass spectrometers are ever-increasingly powerful, user-friendly, and affordable. Thus, the addition of mass spectrometry experiments into the undergraduate laboratory curriculum is now both feasible and an effective tool to introduce students to relevant instrumentation. Here an experiment demonstrating the use of a high-resolution electrospray…

We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude…

Metabolomics is the data-driven science of small molecules. Untargeted metabolomics, using liquid chromatography-high resolution mass spectrometry (LC-HRMS), differs greatly from targeted metabolite assays, using nominal mass LC-MS instruments, as it generates thousands of metabolite features which are not quantified and are identified "post…

The undergraduate biochemistry laboratory curriculum is designed to provide students with experience in protein isolation and purification protocols as well as various data analysis techniques, which enhance the biochemistry lecture course and give students a broad range of tools upon which to build in graduate level laboratories or once they…